Mesenchymal stern cell intracellular labeling using silica-coated superparamagnetic iron oxide nanoparticles with amine functional peripheries

Hao Hao Wang; Yi Xiang J. Wang; Ken Cham Fai Leung; Hui Sheng; Ge Zhang; Simon K.M. Lee; Doris W.T. Au; Chun Pong Chak; Ling Qin; James F. Griffith

doi:10.1109/ITAB.2008.4570593

Mesenchymal stern cell intracellular labeling using silica-coated superparamagnetic iron oxide nanoparticles with amine functional peripheries

Hao Hao Wang, Yi Xiang J. Wang^*, Ken Cham Fai Leung, Hui Sheng, Ge Zhang, Simon K.M. Lee, Doris W.T. Au, Chun Pong Chak, Ling Qin, James F. Griffith

^*Corresponding author for this work

Department of Chemistry

Research output: Chapter in book/report/conference proceeding › Conference proceeding › peer-review

8 Citations (Scopus)

Abstract

Two types of superparamagnetic iron oxide nanoparticles designed for mesenchymal stem cell labeling and magnetic resonance imaging in vivo tracking were constructed in our laboratories. Both types of nanoparticles have an iron oxide core and a thin layer of silica coating, one of them has additional surface modification with amine. The overall particle size was around 8 nanometers. The in vitro study results demonstrated that, without the application of a transfection agent, both nanoparticles could be incorporated into bone marrow derived mesenchymal stem cells and located in lysosomes. Surface modification with amine significantly increased mesenchymal stem cell labeling efficiency, leading to an increased magnetic resonance imaging detection sensitivity of 2-5 folds.

Original language	English
Title of host publication	Proceedings of the 5th International Conference on Information Technology and Application in Biomedicine, in conjunction with The 2nd International Symposium & Summer School on Biomedical and Health Engineering
Publisher	IEEE
Pages	187-189
Number of pages	3
ISBN (Electronic)	9781424422555
ISBN (Print)	9781424422548
DOIs	https://doi.org/10.1109/ITAB.2008.4570593
Publication status	Published - May 2008
Event	5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008: Innovative Technologies for Low-cost Healthcare - Sheraton Shenzhen Futian Hotel and Golden Central Hotel, Shenzhen, China Duration: 30 May 2008 → 31 May 2008 https://ieeexplore.ieee.org/servlet/opac?punumber=4562568

Conference

Conference	5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008
Country/Territory	China
City	Shenzhen
Period	30/05/08 → 31/05/08
Internet address	https://ieeexplore.ieee.org/servlet/opac?punumber=4562568

Scopus Subject Areas

Computer Science(all)
Biomedical Engineering
Health Informatics

User-Defined Keywords

Magnetic resonance imaging
Molecular imaging
Nanoparticles
Stem cell

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10.1109/ITAB.2008.4570593

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Wang, H. H., Wang, Y. X. J., Leung, K. C. F., Sheng, H., Zhang, G., Lee, S. K. M., Au, D. W. T., Chak, C. P., Qin, L., & Griffith, J. F. (2008). Mesenchymal stern cell intracellular labeling using silica-coated superparamagnetic iron oxide nanoparticles with amine functional peripheries. In Proceedings of the 5th International Conference on Information Technology and Application in Biomedicine, in conjunction with The 2nd International Symposium & Summer School on Biomedical and Health Engineering (pp. 187-189). IEEE. https://doi.org/10.1109/ITAB.2008.4570593

Wang, Hao Hao ; Wang, Yi Xiang J. ; Leung, Ken Cham Fai et al. / Mesenchymal stern cell intracellular labeling using silica-coated superparamagnetic iron oxide nanoparticles with amine functional peripheries. Proceedings of the 5th International Conference on Information Technology and Application in Biomedicine, in conjunction with The 2nd International Symposium & Summer School on Biomedical and Health Engineering. IEEE, 2008. pp. 187-189

@inproceedings{b7adc17875c34f9884a3f3a7ef7449e9,

title = "Mesenchymal stern cell intracellular labeling using silica-coated superparamagnetic iron oxide nanoparticles with amine functional peripheries",

abstract = "Two types of superparamagnetic iron oxide nanoparticles designed for mesenchymal stem cell labeling and magnetic resonance imaging in vivo tracking were constructed in our laboratories. Both types of nanoparticles have an iron oxide core and a thin layer of silica coating, one of them has additional surface modification with amine. The overall particle size was around 8 nanometers. The in vitro study results demonstrated that, without the application of a transfection agent, both nanoparticles could be incorporated into bone marrow derived mesenchymal stem cells and located in lysosomes. Surface modification with amine significantly increased mesenchymal stem cell labeling efficiency, leading to an increased magnetic resonance imaging detection sensitivity of 2-5 folds.",

keywords = "Magnetic resonance imaging, Molecular imaging, Nanoparticles, Stem cell",

author = "Wang, {Hao Hao} and Wang, {Yi Xiang J.} and Leung, {Ken Cham Fai} and Hui Sheng and Ge Zhang and Lee, {Simon K.M.} and Au, {Doris W.T.} and Chak, {Chun Pong} and Ling Qin and Griffith, {James F.}",

note = "Funding information: This work was supported by the Innovative Technology Commission of Hong Kong SAR (Ref: ITS/016/07). Publisher copyright: {\textcopyright}2008 IEEE; 5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008 : Innovative Technologies for Low-cost Healthcare ; Conference date: 30-05-2008 Through 31-05-2008",

year = "2008",

month = may,

doi = "10.1109/ITAB.2008.4570593",

language = "English",

isbn = "9781424422548",

pages = "187--189",

booktitle = "Proceedings of the 5th International Conference on Information Technology and Application in Biomedicine, in conjunction with The 2nd International Symposium & Summer School on Biomedical and Health Engineering",

publisher = "IEEE",

address = "United States",

url = "https://ieeexplore.ieee.org/servlet/opac?punumber=4562568",

}

Wang, HH, Wang, YXJ, Leung, KCF, Sheng, H, Zhang, G, Lee, SKM, Au, DWT, Chak, CP, Qin, L & Griffith, JF 2008, Mesenchymal stern cell intracellular labeling using silica-coated superparamagnetic iron oxide nanoparticles with amine functional peripheries. in Proceedings of the 5th International Conference on Information Technology and Application in Biomedicine, in conjunction with The 2nd International Symposium & Summer School on Biomedical and Health Engineering. IEEE, pp. 187-189, 5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008, Shenzhen, China, 30/05/08. https://doi.org/10.1109/ITAB.2008.4570593

Mesenchymal stern cell intracellular labeling using silica-coated superparamagnetic iron oxide nanoparticles with amine functional peripheries. / Wang, Hao Hao; Wang, Yi Xiang J.; Leung, Ken Cham Fai et al.
Proceedings of the 5th International Conference on Information Technology and Application in Biomedicine, in conjunction with The 2nd International Symposium & Summer School on Biomedical and Health Engineering. IEEE, 2008. p. 187-189.

Research output: Chapter in book/report/conference proceeding › Conference proceeding › peer-review

TY - GEN

T1 - Mesenchymal stern cell intracellular labeling using silica-coated superparamagnetic iron oxide nanoparticles with amine functional peripheries

AU - Wang, Hao Hao

AU - Wang, Yi Xiang J.

AU - Leung, Ken Cham Fai

AU - Sheng, Hui

AU - Zhang, Ge

AU - Lee, Simon K.M.

AU - Au, Doris W.T.

AU - Chak, Chun Pong

AU - Qin, Ling

AU - Griffith, James F.

PY - 2008/5

Y1 - 2008/5

N2 - Two types of superparamagnetic iron oxide nanoparticles designed for mesenchymal stem cell labeling and magnetic resonance imaging in vivo tracking were constructed in our laboratories. Both types of nanoparticles have an iron oxide core and a thin layer of silica coating, one of them has additional surface modification with amine. The overall particle size was around 8 nanometers. The in vitro study results demonstrated that, without the application of a transfection agent, both nanoparticles could be incorporated into bone marrow derived mesenchymal stem cells and located in lysosomes. Surface modification with amine significantly increased mesenchymal stem cell labeling efficiency, leading to an increased magnetic resonance imaging detection sensitivity of 2-5 folds.

AB - Two types of superparamagnetic iron oxide nanoparticles designed for mesenchymal stem cell labeling and magnetic resonance imaging in vivo tracking were constructed in our laboratories. Both types of nanoparticles have an iron oxide core and a thin layer of silica coating, one of them has additional surface modification with amine. The overall particle size was around 8 nanometers. The in vitro study results demonstrated that, without the application of a transfection agent, both nanoparticles could be incorporated into bone marrow derived mesenchymal stem cells and located in lysosomes. Surface modification with amine significantly increased mesenchymal stem cell labeling efficiency, leading to an increased magnetic resonance imaging detection sensitivity of 2-5 folds.

KW - Magnetic resonance imaging

KW - Molecular imaging

KW - Nanoparticles

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BT - Proceedings of the 5th International Conference on Information Technology and Application in Biomedicine, in conjunction with The 2nd International Symposium & Summer School on Biomedical and Health Engineering

PB - IEEE

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Wang HH, Wang YXJ, Leung KCF, Sheng H, Zhang G, Lee SKM et al. Mesenchymal stern cell intracellular labeling using silica-coated superparamagnetic iron oxide nanoparticles with amine functional peripheries. In Proceedings of the 5th International Conference on Information Technology and Application in Biomedicine, in conjunction with The 2nd International Symposium & Summer School on Biomedical and Health Engineering. IEEE. 2008. p. 187-189 doi: 10.1109/ITAB.2008.4570593

Mesenchymal stern cell intracellular labeling using silica-coated superparamagnetic iron oxide nanoparticles with amine functional peripheries

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