TY - JOUR
T1 - Mechanistic Insights and Rational Design of a Versatile Surface with Cells/Bacteria Recognition Capability via Orientated Fusion Peptides
AU - Wang, Lin
AU - Chen, Junjian
AU - Zeng, Xiangze
AU - Cheung, Peter Pak Hang
AU - Zheng, Xiaoyan
AU - Xie, Liangxu
AU - Shi, Xuetao
AU - Ren, Li
AU - Huang, Xuhui
AU - Wang, Yingjun
N1 - Funding Information:
L.W. and J.C. contributed equally to this work. The authors thank the National Key R&D Program of China (Grant No. 2018YFC1105402), the National Nature Science Foundation of China (Grant Nos. 31771027 and U1801252), the Hong Kong Scholars Program (Grant No. XJ2015016), the Innovation and Technology Commission (Grant No. ITC-CNERC14SC01), Science and Technology Program of Guangzhou (Grant No. 201804020060), Pearl River Nova Program of Guangzhou (Grant No. 201806010156), National Key Research and Development Program (Grant No. 2017YFC1104402), Hong Kong Research Grant Council (Grant Nos. HKUST C6009-15G, 16305817, 16302214, 16318816, and AoE/P-705/16), and Shenzhen Science and Technology Innovation Committee (Grant No. JCYJ20170413173837121). X.H. is the Padma Harilela Associate Professor of Science. The authors thank Ilona Christy Unarta, Dr. Siqin Cao, and Dr. Junhui Peng for the helpful discussion.
Publisher Copyright:
© 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/5/3
Y1 - 2019/5/3
N2 - Hospital-acquired infection causes many deaths worldwide and calls for the urgent need for antibacterial biomaterials used in clinic that can selectively kill harmful bacteria. The present study rationally designs fusion peptides capable of undergoing 2D self-assembly on the poly(methyl methacrylate) surface to form a smart surface, which can maintain a desirable orientation via electrostatic interactions. The in vitro assay shows that the smart surface can recognize bacteria to exert antibacterial activity and is nontoxic toward mouse bone mesenchymal stem cells. Excitingly, the smart surface can distinguish different bacterial strains. This selective feature, from being broad-spectrum to being highly selective against S. aureus, can be altered by varying the number of amino acids in the recognition sequences. By all-atom molecular dynamics simulations, it is also found that the recognition sequence in the peptide is critical for the selectivity toward specific bacterial strains, in which a less accessible surface area for the bacteria in the antimicrobial peptide sequence is responsible for such selectivity. Finally, the smart surface can inhibit S. aureus infection in vivo with much more rapid tissue-healing compared to the control.
AB - Hospital-acquired infection causes many deaths worldwide and calls for the urgent need for antibacterial biomaterials used in clinic that can selectively kill harmful bacteria. The present study rationally designs fusion peptides capable of undergoing 2D self-assembly on the poly(methyl methacrylate) surface to form a smart surface, which can maintain a desirable orientation via electrostatic interactions. The in vitro assay shows that the smart surface can recognize bacteria to exert antibacterial activity and is nontoxic toward mouse bone mesenchymal stem cells. Excitingly, the smart surface can distinguish different bacterial strains. This selective feature, from being broad-spectrum to being highly selective against S. aureus, can be altered by varying the number of amino acids in the recognition sequences. By all-atom molecular dynamics simulations, it is also found that the recognition sequence in the peptide is critical for the selectivity toward specific bacterial strains, in which a less accessible surface area for the bacteria in the antimicrobial peptide sequence is responsible for such selectivity. Finally, the smart surface can inhibit S. aureus infection in vivo with much more rapid tissue-healing compared to the control.
KW - antibacterial activity
KW - fusion peptides
KW - molecular dynamics simulation
KW - solvent accessible surface area
KW - surface modification
UR - http://www.scopus.com/inward/record.url?scp=85062370513&partnerID=8YFLogxK
U2 - 10.1002/advs.201801827
DO - 10.1002/advs.201801827
M3 - Journal article
AN - SCOPUS:85062370513
SN - 2198-3844
VL - 6
JO - Advanced Science
JF - Advanced Science
IS - 9
M1 - 1801827
ER -