Mechanism of non-capacitative Ca2+ influx in response to bradykinin in vascular endothelial cells

Pan Cheung Leung, Kwong Tai Cheng, Cuiling Liu, Wing Tai Cheung, Hiu Yee Kwan, Kin Ling Lau, Yu Huang, Xiaoqiang Yao*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

38 Citations (Scopus)


Bradykinin is a potent vasoactive nonapeptide. It elicits a rise in cytosolic Ca2+ (Ca2+)i in endothelial cells, resulting in Ca2+-dependent synthesis and release of endothelial vasodilators. In the present study, we investigated the mechanism of bradykinin-induced Ca2+ influx in primary cultured rat aortic endothelial cells and in a mouse heart microvessel endothelial cell line (H5V). Bradykinin-induced Ca2+ influx was resolved into capacitative Ca 2+ entry (CCE) and non-CCE. The non-CCE component was inhibited by a B2 receptor antagonist (HOE140; 1 μM) and a phospholipase C (PLC) inhibitor (U73122; 10 μM). The action of bradykinin could be mimicked by 1-oleoyl-2-acetyl-glycerol, an analogue of diacylglycerol (DAG), and by RHC80267, a DAG-lipase inhibitor. Immunoblots showed that TRPC6 was one of the main TRPC channels expressed in endothelial cells. Transfection of H5V cells with two siRNA constructs against TRPC6 both markedly reduced the TRPC6 protein level and, at the same time, decreased the percentage of cells displaying bradykinin-induced non-CCE. siRNA transfection also reduced the magnitude of non-CCE among the cells responding to bradykinin. Taken together, our data suggest that bradykinin-induced non-CCE is mediated via the B2-PLC pathway, and that DAG may be involved in this process. Further, TRPC6 is one of the important channels participating in bradykinin-induced non-CCE in endothelial cells.

Original languageEnglish
Pages (from-to)367-376
Number of pages10
JournalJournal of Vascular Research
Issue number4
Publication statusPublished - Jul 2006

Scopus Subject Areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

User-Defined Keywords

  • Bradykinin
  • Diacylglycerol
  • Non-capacitative Ca entry
  • Phospholipase C
  • TRPC6


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