Mechanism by which Eucommia ulmoides leaves Regulate Nonalcoholic fatty liver disease based on system pharmacology

Man Gong, Chengfu Su, Mengzhe Fan, Ping Wang, Bingdi Cui, Zhongyuan Guo, Shaojia Liang, Lianhe Yang, Xiaoqian Liu, Liping Dai*, Zhimin Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Ethnopharmacological relevance

Eucommia ulmoides (E. ulmoides) leaves are included in the Chinese Pharmacopoeia, and are traditionally used to treat hypertension, obesity, diabetes, and other diseases. Numerous pharmacological studies have shown that E. ulmoides has a good effect on lowering blood lipids and can improve obesity and nonalcoholic fatty liver. 

Aim

To study the mechanism of E. ulmoides leaves in regulating nonalcoholic fatty liver disease by combining prediction and validation. 

Methods

Using network pharmacology, and molecular docking to predict E. ulmoides in regulating the action mechanism and potential active ingredients of nonalcoholic fatty liver, large hole adsorption resin enrichment active sites, in vitro experiments were performed to verify its fat-lowering effect and mechanism. 

Results

The major components of E. ulmoides leaves exhibited good combination with lipid metabolism-regulating core proteins, particularly flavonoids. EUL 50 significantly reduced lipid accumulation, and increased PPARγ. Compared with the control group, the autophagy level increased after the administration of EUL 50. PPARγ decreased significantly after the addition of chloroquine (CQ, autophagy inhibitor). 

Conclusion

The active ingredients in E. ulmoides leaves regulating nonalcoholic fatty liver disease are mainly flavonoids and phenolics. EUL 50 may play a role in lowering lipids by regulating PPARγ expression through inducing autophagy.

Original languageEnglish
Article number114603
JournalJournal of Ethnopharmacology
Volume282
Early online date5 Sep 2021
DOIs
Publication statusPublished - 10 Jan 2022

Scopus Subject Areas

  • Pharmacology
  • Drug Discovery

User-Defined Keywords

  • Autophagy
  • Eucommia ulmoides leaves
  • Flavonoids
  • Nonalcoholic fatty liver disease
  • Nuclear receptors
  • Phenolics

Fingerprint

Dive into the research topics of 'Mechanism by which Eucommia ulmoides leaves Regulate Nonalcoholic fatty liver disease based on system pharmacology'. Together they form a unique fingerprint.

Cite this