TY - JOUR
T1 - Mass spectrometry investigation of nucleoside adducts of fatty acid hydroperoxides from oxidation of linolenic and linoleic acids
AU - Cao, Guodong
AU - Ding, Cheng
AU - Yang, Zhiyi
AU - Wu, Pengfei
AU - Lu, Minghua
AU - Guo, Jinggong
AU - Chen, Xiangfeng
AU - Hong, Yanjun
AU - Cai, Zongwei
N1 - The authors acknowledge the National Key Research and Development Program of China ( 2017YFC1600500 ), National Nature Science Foundation of China (21575120 & 21707112), and Hong Kong General Research Fund (12302317).
Publisher Copyright:
© 2021
PY - 2021/7/19
Y1 - 2021/7/19
N2 - The widespread presence of lipid hydroperoxides in foodstuffs and biological samples has aroused great attentions in recent years, while it remains challenging for analysis of the fragility of O − O bond linkage of peroxides. In this present study, we explored the utility of electrospray ionization mass spectrometry (ESI-MS) for characterization of two fatty acid hydroperoxides from oxidation of linoleic acid and α-linolenic acid, which are the essential fatty acids abundant in many seeds and vegetable oils. The results indicated that in-source fragmentation occurred in the detection of the two fatty acid hydroperoxides in both positive and negative ion modes, which yielded characteristic fragments for ESI-MS analysis. In addition, the genotoxicity of fatty acid hydroperoxides for generation of nucleoside adducts was investigated. It was found that a variety of nucleoside adducts were formed from the reactions of fatty acid hydroperoxides and nucleosides. Furthermore, the decomposition products of the fatty acid hydroperoxides were determined, which provided evidence to elucidate the reaction mechanism for formation of nucleoside adducts.
AB - The widespread presence of lipid hydroperoxides in foodstuffs and biological samples has aroused great attentions in recent years, while it remains challenging for analysis of the fragility of O − O bond linkage of peroxides. In this present study, we explored the utility of electrospray ionization mass spectrometry (ESI-MS) for characterization of two fatty acid hydroperoxides from oxidation of linoleic acid and α-linolenic acid, which are the essential fatty acids abundant in many seeds and vegetable oils. The results indicated that in-source fragmentation occurred in the detection of the two fatty acid hydroperoxides in both positive and negative ion modes, which yielded characteristic fragments for ESI-MS analysis. In addition, the genotoxicity of fatty acid hydroperoxides for generation of nucleoside adducts was investigated. It was found that a variety of nucleoside adducts were formed from the reactions of fatty acid hydroperoxides and nucleosides. Furthermore, the decomposition products of the fatty acid hydroperoxides were determined, which provided evidence to elucidate the reaction mechanism for formation of nucleoside adducts.
KW - Fatty acid hydroperoxides
KW - Mass spectrometry
KW - Nucleoside adducts
KW - Vegetable oil
KW - α, β-unsaturated aldehydes
UR - http://www.scopus.com/inward/record.url?scp=85106382639&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2021.462236
DO - 10.1016/j.chroma.2021.462236
M3 - Journal article
C2 - 34038777
AN - SCOPUS:85106382639
SN - 0021-9673
VL - 1649
JO - Journal of Chromatography A
JF - Journal of Chromatography A
M1 - 462236
ER -
