Mass spectrometry-based lipidomics analysis using methyl tert-butyl ether extraction in human hepatocellular carcinoma tissues

Shuxia Jiang; Yongle Li; Shuhai Lin; Hongbo Yang; Xin Yuan Guan; Haiyun Zhou; Tiangang Luan; Zongwei Cai

doi:10.1039/c5ay01413a

Mass spectrometry-based lipidomics analysis using methyl tert-butyl ether extraction in human hepatocellular carcinoma tissues

Shuxia Jiang, Yongle Li, Shuhai Lin, Hongbo Yang, Xin Yuan Guan, Haiyun Zhou^*, Tiangang Luan, Zongwei Cai^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

6 Citations (Scopus)

Abstract

Hepatocellular carcinoma (HCC) is one of the most common cancers in human beings and not well treated. Due to its blurred biomarkers, lipidomics can be considered as a new diagnostic tool. In this study, mass spectrometry-based lipidomics analysis coupled with multivariate statistical analysis was applied to profile twelve pairs of human hepatocellular carcinoma tissues (HCT) and matched adjacent non-tumour tissues (ANT). As a result, perturbation of lipid biosynthesis was observed in HCT compared to ANT. Lipid species were profiled and 28 significant lipids were identified based on high resolution mass spectrometry with mass error less than 3 ppm, covering phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), sphingomyelin (SM) and triglyceride (TG). The high mass accuracy as well as the tandem MS (MS/MS) technique provides high confidence of lipid identification. Interestingly, decreased PGs and PIs were observed in this study, indicating that lipolysis of PGs and PIs might play a crucial role in HCC development. The results indicated that mass spectrometry-based lipidomics analysis could serve as an effective means to diagnose canceration.

Original language	English
Pages (from-to)	8466-8471
Number of pages	6
Journal	Analytical Methods
Volume	7
Issue number	19
Early online date	26 Aug 2015
DOIs	https://doi.org/10.1039/c5ay01413a
Publication status	Published - 7 Oct 2015

Scopus Subject Areas

Analytical Chemistry
Chemical Engineering(all)
Engineering(all)

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title = "Mass spectrometry-based lipidomics analysis using methyl tert-butyl ether extraction in human hepatocellular carcinoma tissues",

abstract = "Hepatocellular carcinoma (HCC) is one of the most common cancers in human beings and not well treated. Due to its blurred biomarkers, lipidomics can be considered as a new diagnostic tool. In this study, mass spectrometry-based lipidomics analysis coupled with multivariate statistical analysis was applied to profile twelve pairs of human hepatocellular carcinoma tissues (HCT) and matched adjacent non-tumour tissues (ANT). As a result, perturbation of lipid biosynthesis was observed in HCT compared to ANT. Lipid species were profiled and 28 significant lipids were identified based on high resolution mass spectrometry with mass error less than 3 ppm, covering phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), sphingomyelin (SM) and triglyceride (TG). The high mass accuracy as well as the tandem MS (MS/MS) technique provides high confidence of lipid identification. Interestingly, decreased PGs and PIs were observed in this study, indicating that lipolysis of PGs and PIs might play a crucial role in HCC development. The results indicated that mass spectrometry-based lipidomics analysis could serve as an effective means to diagnose canceration.",

author = "Shuxia Jiang and Yongle Li and Shuhai Lin and Hongbo Yang and Guan, {Xin Yuan} and Haiyun Zhou and Tiangang Luan and Zongwei Cai",

note = "Funding information: The authors would like to acknowledge financial support from the Collaborative Research Fund (HKBU5/CRF/10) of Hong Kong Research Grant Council and National Natural Science Foundation of China (NSFC-21377106). Publisher copyright: {\textcopyright} The Royal Society of Chemistry 2015",

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T1 - Mass spectrometry-based lipidomics analysis using methyl tert-butyl ether extraction in human hepatocellular carcinoma tissues

AU - Jiang, Shuxia

AU - Li, Yongle

AU - Lin, Shuhai

AU - Yang, Hongbo

AU - Guan, Xin Yuan

AU - Zhou, Haiyun

AU - Luan, Tiangang

AU - Cai, Zongwei

N1 - Funding information: The authors would like to acknowledge financial support from the Collaborative Research Fund (HKBU5/CRF/10) of Hong Kong Research Grant Council and National Natural Science Foundation of China (NSFC-21377106). Publisher copyright: © The Royal Society of Chemistry 2015

PY - 2015/10/7

Y1 - 2015/10/7

N2 - Hepatocellular carcinoma (HCC) is one of the most common cancers in human beings and not well treated. Due to its blurred biomarkers, lipidomics can be considered as a new diagnostic tool. In this study, mass spectrometry-based lipidomics analysis coupled with multivariate statistical analysis was applied to profile twelve pairs of human hepatocellular carcinoma tissues (HCT) and matched adjacent non-tumour tissues (ANT). As a result, perturbation of lipid biosynthesis was observed in HCT compared to ANT. Lipid species were profiled and 28 significant lipids were identified based on high resolution mass spectrometry with mass error less than 3 ppm, covering phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), sphingomyelin (SM) and triglyceride (TG). The high mass accuracy as well as the tandem MS (MS/MS) technique provides high confidence of lipid identification. Interestingly, decreased PGs and PIs were observed in this study, indicating that lipolysis of PGs and PIs might play a crucial role in HCC development. The results indicated that mass spectrometry-based lipidomics analysis could serve as an effective means to diagnose canceration.

AB - Hepatocellular carcinoma (HCC) is one of the most common cancers in human beings and not well treated. Due to its blurred biomarkers, lipidomics can be considered as a new diagnostic tool. In this study, mass spectrometry-based lipidomics analysis coupled with multivariate statistical analysis was applied to profile twelve pairs of human hepatocellular carcinoma tissues (HCT) and matched adjacent non-tumour tissues (ANT). As a result, perturbation of lipid biosynthesis was observed in HCT compared to ANT. Lipid species were profiled and 28 significant lipids were identified based on high resolution mass spectrometry with mass error less than 3 ppm, covering phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), sphingomyelin (SM) and triglyceride (TG). The high mass accuracy as well as the tandem MS (MS/MS) technique provides high confidence of lipid identification. Interestingly, decreased PGs and PIs were observed in this study, indicating that lipolysis of PGs and PIs might play a crucial role in HCC development. The results indicated that mass spectrometry-based lipidomics analysis could serve as an effective means to diagnose canceration.

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DO - 10.1039/c5ay01413a

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JO - Analytical Methods

JF - Analytical Methods

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Mass spectrometry-based lipidomics analysis using methyl tert-butyl ether extraction in human hepatocellular carcinoma tissues

Abstract

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