Manipulation of the induction of adjuvant arthritis in Sprague-Dawley rats

X. Cai, Y. F. Wong, H. Zhou, Q. Liu, Y. Xie, Zhi Hong JIANG, Zhaoxiang BIAN, H. X. Xu, L. Liu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Objective: To investigate the roles of various variables in the induction of adjuvant-induced arthritis (AIA) in the outbred Sprague-Dawley (SD) rats, and further characterize its arthritic features by comprehensive examinations. Methods: The roles of different preparative techniques, inoculation routes and doses of Mycobacterium tuberculosis (MT) suspension as well as the sex preference in the induction of AIA were comparatively studied using clinical assessment. The hind paws of animals were analyzed by radiological and histological examinations. The serum levels of cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α were determined by ELISA. Results: The particle size and dose of MT played a dominant role in the induction and severity of AIA. Male rats manifested markedly more severe arthritic signs than female rats. After subcutaneously inoculated with 500 μg MT, male rats developed pronounced arthritis with 100% incidence and low variable clinical signs. Even using only 62.5 μg MT, AIA was efficiently induced in male rats and characterized by up-regulated expression profiles of IL-1β, IL-6 and TNF-α. Conclusions: Since outbred SD rats are much cheaper and more readily available than Lewis rats, this well-developed SD rat AIA model is an efficient and cost-effective arthritis model available for screening novel anti-arthritic agents.

Original languageEnglish
Pages (from-to)368-377
Number of pages10
JournalInflammation Research
Volume55
Issue number9
DOIs
Publication statusPublished - Sep 2006

Scopus Subject Areas

  • Immunology
  • Pharmacology

User-Defined Keywords

  • Adjuvant-induced arthritis
  • Mycobacterium tuberculosis
  • Pro-inflammatory cytokines
  • Sprague-Dawley rats

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