TY - JOUR
T1 - Manipulation of the induction of adjuvant arthritis in Sprague-Dawley rats
AU - Cai, X.
AU - Wong, Y. F.
AU - Zhou, H.
AU - Liu, Q.
AU - Xie, Y.
AU - JIANG, Zhi Hong
AU - BIAN, Zhaoxiang
AU - Xu, H. X.
AU - Liu, L.
N1 - Funding Information:
Acknowledgements. This research was funded by the Hong Kong Jockey Club Charities Trust. We are grateful to Profs. Biao Yan Du, Pei Xun Wang and Liang Zhou of the Guangzhou University of Traditional Chinese Medicine (GUTCM) for their assistance in histological analysis, and to Ms. Rui Yan Liang of GUTCM for her assistance in radiographic analysis. We also thank Dr. Martha Dahlen for her excellent language editing of the manuscript.
PY - 2006/9
Y1 - 2006/9
N2 - Objective: To investigate the roles of various variables in the induction of adjuvant-induced arthritis (AIA) in the outbred Sprague-Dawley (SD) rats, and further characterize its arthritic features by comprehensive examinations. Methods: The roles of different preparative techniques, inoculation routes and doses of Mycobacterium tuberculosis (MT) suspension as well as the sex preference in the induction of AIA were comparatively studied using clinical assessment. The hind paws of animals were analyzed by radiological and histological examinations. The serum levels of cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α were determined by ELISA. Results: The particle size and dose of MT played a dominant role in the induction and severity of AIA. Male rats manifested markedly more severe arthritic signs than female rats. After subcutaneously inoculated with 500 μg MT, male rats developed pronounced arthritis with 100% incidence and low variable clinical signs. Even using only 62.5 μg MT, AIA was efficiently induced in male rats and characterized by up-regulated expression profiles of IL-1β, IL-6 and TNF-α. Conclusions: Since outbred SD rats are much cheaper and more readily available than Lewis rats, this well-developed SD rat AIA model is an efficient and cost-effective arthritis model available for screening novel anti-arthritic agents.
AB - Objective: To investigate the roles of various variables in the induction of adjuvant-induced arthritis (AIA) in the outbred Sprague-Dawley (SD) rats, and further characterize its arthritic features by comprehensive examinations. Methods: The roles of different preparative techniques, inoculation routes and doses of Mycobacterium tuberculosis (MT) suspension as well as the sex preference in the induction of AIA were comparatively studied using clinical assessment. The hind paws of animals were analyzed by radiological and histological examinations. The serum levels of cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α were determined by ELISA. Results: The particle size and dose of MT played a dominant role in the induction and severity of AIA. Male rats manifested markedly more severe arthritic signs than female rats. After subcutaneously inoculated with 500 μg MT, male rats developed pronounced arthritis with 100% incidence and low variable clinical signs. Even using only 62.5 μg MT, AIA was efficiently induced in male rats and characterized by up-regulated expression profiles of IL-1β, IL-6 and TNF-α. Conclusions: Since outbred SD rats are much cheaper and more readily available than Lewis rats, this well-developed SD rat AIA model is an efficient and cost-effective arthritis model available for screening novel anti-arthritic agents.
KW - Adjuvant-induced arthritis
KW - Mycobacterium tuberculosis
KW - Pro-inflammatory cytokines
KW - Sprague-Dawley rats
UR - http://www.scopus.com/inward/record.url?scp=35648967927&partnerID=8YFLogxK
U2 - 10.1007/s00011-006-6026-x
DO - 10.1007/s00011-006-6026-x
M3 - Journal article
C2 - 17122958
AN - SCOPUS:35648967927
SN - 1023-3830
VL - 55
SP - 368
EP - 377
JO - Inflammation Research
JF - Inflammation Research
IS - 9
ER -
