Magnolol inhibits colonic motility through down-regulation of voltage-sensitive L-type Ca2+ channels of colonic smooth muscle cells in rats

Man Zhang, Kai Hong Zang, Jia Lie Luo, Fung Ping LEUNG, Yu Huang, Cheng Yuan Lin, Zhijun YANG, Aiping LYU, Xu Dong Tang, Hong Xi Xu, Joseph Jao Yiu Sung, Zhaoxiang BIAN*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

This study aimed to investigate the effect of magnolol (5,5′-diallyl- 2,2′-biphenyldiol) on contraction in distal colonic segments of rats and the underlying mechanisms. Colonic segments were mounted in organ baths for isometric force measurement. Whole-cell voltage-sensitive L-type Ca2+ currents were recorded on isolated single colonic smooth muscle cells using patch-clamp technique. The spontaneous contractions and acetylcholine (ACh)- and Bay K 8644-induced contractions were inhibited by magnolol (3-100 μM). In the presence of Bay K8644 (100 nM), magnolol (10-100 μM) inhibited the contraction induced by 10 μM ACh. By contrast, tetrodotoxin (100 nM) and Nώ-nitro-l-arginine methyl ester (l-NAME 100 μM) did not change the inhibitory effect of magnolol (10 μM). In addition, magnolol (3-100 μM) inhibited the L-type Ca2+ currents. The present results suggest that magnolol inhibits colonic smooth muscle contraction through downregulating L-type Ca2+ channel activity.

Original languageEnglish
Pages (from-to)1272-1279
Number of pages8
JournalPhytomedicine
Volume20
Issue number14
DOIs
Publication statusPublished - 15 Nov 2013

Scopus Subject Areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

User-Defined Keywords

  • Colonic motility
  • L-type Ca channels
  • Magnolia officinalis
  • Magnolol
  • Smooth muscle cells

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