Magnetic solid-phase extraction based on a trimethylstearylammonium bromide coated Fe3O4/SiO2 composite for determination of adriamycin hydrochloride in human plasma and urine by HPLC-FLD

Nengxuan Ma, Lin Zhang, Ruijin Li, Yehong Zhou, Zongwei CAI, Chuan Dong, Shaomin Shuang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A novel trimethylstearylammonium bromide (TSAB) coated Fe3O 4/SiO2 magnetic nanocomposite was fabricated and used as the adsorbent for the magnetic solid-phase extraction of adriamycin hydrochloride (ADR) in human plasma and urine with high performance liquid chromatography-fluorescence detection (HPLC-FLD). The factors influencing the extraction efficiency were examined including the pH value, extraction time, elution solvent, etc. A good linearity was presented by HPLC-FLD in the range of 0.5-10.0 μg mL-1 for ADR, with the correlation coefficient of 0.998 (R2). The relative standard deviation was 4.27%. The limit of detection and limit of quantitation were 5.05 and 16.82 ng mL-1, respectively. The feasibility of the developed method was further validated by extraction of ADR in plasma and urine samples. The recoveries were in the range of 76.5-94.0% and 77.9-96.0% for plasma and urine samples, respectively. This journal is

Original languageEnglish
Pages (from-to)6736-6744
Number of pages9
JournalAnalytical Methods
Volume6
Issue number17
DOIs
Publication statusPublished - 7 Sep 2014

Scopus Subject Areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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