Luteolin exerted less inhibitory effect on macrophage activation induced by Astragalus polysaccharide than by lipopolysaccharide

Zhipeng LI, Quan Wei Zhang, Wei Wei, Li Feng Li, Edmond Dik Lung MA, Chung Hang Leung, Aiping LYU, Zhaoxiang BIAN*, Simon Q B HAN

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

As a kind of food supplementary, polysaccharide-rich tonic herbs become more and more popular. However, little is known regarding the interaction between known active molecules with these polysaccharides. This study aims to investigate effects of luteolin on RAW264.7 cells induced by Astragalus polysaccharide RAP in comparison with lipopolysaccharide (LPS). The results showed that luteolin suppressed inflammatory meditors in LPS-induced RAW264.7 cells. While in the RAP-induced RAW264.7 cells, luteolin only decreased the NO production and iNOS gene expression, but only inhibit IL-6 at high concentration, and was ineffective in term of TNF-α. The results of western blot analysis suggested that luteolin only inhibited the phosphorylation of NF-κB p65 and Akt, but not MAPKs and IRF3 in RAP-induced RAW264.7 cells. Taken together, lutelion exerted less inhibitory effect on macrophage induced by RAP than by LPS. This study demonstrated the luteolin-containing foods may not strongly interfere the immunoregulation effect of polysaccharide RAP.

Original languageEnglish
Pages (from-to)618-623
Number of pages6
JournalJournal of Functional Foods
Volume37
DOIs
Publication statusPublished - Oct 2017

Scopus Subject Areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

User-Defined Keywords

  • Astragalus polysaccharide
  • Luteolin
  • MAPKs
  • NF-κB
  • RAW264.7

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