Luminescent Strategies for Label-Free G-Quadruplex-Based Enzyme Activity Sensing

Edmond Dik Lung MA*, Zhen Zhen Dong, Kasipandi Vellaisamy, Ka Man Cheung, Guanjun Yang, Chung Hang Leung

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

7 Citations (Scopus)

Abstract

By catalyzing highly specific and tightly controlled chemical reactions, enzymes are essential to maintaining normal cellular physiology. However, aberrant enzymatic activity can be linked to the pathogenesis of various diseases. Therefore, the unusual activity of particular enzymes can represent testable biomarkers for the diagnosis or screening of certain diseases. In recent years, G-quadruplex-based platforms have attracted wide attention for the monitoring of enzymatic activities. In this Personal Account, we discuss our group's works on the development of G-quadruplex-based sensing system for enzyme activities by using mainly iridium(III) complexes as luminescent label-free probes. These studies showcase the versatility of the G-quadruplex for developing assays for a variety of different enzymes.

Original languageEnglish
Pages (from-to)1135-1145
Number of pages11
JournalChemical Record
Volume17
Issue number11
DOIs
Publication statusPublished - Nov 2017

Scopus Subject Areas

  • Chemistry(all)
  • Biochemistry
  • Chemical Engineering(all)
  • Materials Chemistry

User-Defined Keywords

  • enzyme
  • G-quadruplex
  • iridium(III) complex
  • luminescent enhancement

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