Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution

Zhen Zhen Dong; Lihua Lu; Wanhe Wang; Guodong Li; Tian Shu Kang; Simon Q B Han; Chung Hang Leung; Edmond Dik Lung Ma

doi:10.1016/j.snb.2017.02.156

Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution

Zhen Zhen Dong, Lihua Lu, Wanhe Wang, Guodong Li, Tian Shu Kang, Simon Q B Han, Chung Hang Leung^*, Edmond Dik Lung Ma

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

12 Citations (Scopus)

Abstract

A cyclometalated iridium(III) complex has been synthesized as a novel luminescent probe for G-quadruplex (G4) DNA in aqueous solution, and a G4-based assay was employed for the detection of nicking endonuclease activity using Nb.BsmI as a model enzyme. In the assay, an initial hairpin DNA (5′- TG₄AG₃TG₄AG₃TG₄A₂G₃A₂TGCTA₃TAGCAT₂C₃T₂C₄AC-3′) sequence containing the Nb.BsmI recognition site in its stem region and a partially caged G-rich DNA sequence at its 5′-terminus could be cleaved by Nb.BsmI, which liberates the G-rich DNA sequence and enhances the luminescence of complex 1. This assay showed a linear relation between luminescent signal and Nb.BsmI concentration over the range of 0–10 U/mL (R² = 0.9937) with a detection limit for Nb.BsmI of 0.042 U/mL. Furthermore, this assay showed high selectivity for Nb.BsmI over other nicking endonucleases, and functioned effectively in the presence of cell lysates.

Original language	English
Pages (from-to)	826-832
Number of pages	7
Journal	Sensors and Actuators B: Chemical
Volume	246
DOIs	https://doi.org/10.1016/j.snb.2017.02.156
Publication status	Published - Jul 2017

Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

User-Defined Keywords

G-quadruplex
Iridium(III) complex
Luminescence
Nicking endonuclease
Probe

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10.1016/j.snb.2017.02.156

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@article{0498743a4bb34ad49ac305c939ccec4e,

title = "Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution",

abstract = "A cyclometalated iridium(III) complex has been synthesized as a novel luminescent probe for G-quadruplex (G4) DNA in aqueous solution, and a G4-based assay was employed for the detection of nicking endonuclease activity using Nb.BsmI as a model enzyme. In the assay, an initial hairpin DNA (5′- TG4AG3TG4AG3TG4A2G3A2TGCTA3TAGCAT2C3T2C4AC-3′) sequence containing the Nb.BsmI recognition site in its stem region and a partially caged G-rich DNA sequence at its 5′-terminus could be cleaved by Nb.BsmI, which liberates the G-rich DNA sequence and enhances the luminescence of complex 1. This assay showed a linear relation between luminescent signal and Nb.BsmI concentration over the range of 0–10 U/mL (R2 = 0.9937) with a detection limit for Nb.BsmI of 0.042 U/mL. Furthermore, this assay showed high selectivity for Nb.BsmI over other nicking endonucleases, and functioned effectively in the presence of cell lysates.",

keywords = "G-quadruplex, Iridium(III) complex, Luminescence, Nicking endonuclease, Probe",

author = "Dong, {Zhen Zhen} and Lihua Lu and Wanhe Wang and Guodong Li and Kang, {Tian Shu} and Han, {Simon Q B} and Leung, {Chung Hang} and Ma, {Edmond Dik Lung}",

year = "2017",

month = jul,

doi = "10.1016/j.snb.2017.02.156",

language = "English",

volume = "246",

pages = "826--832",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier",

}

TY - JOUR

T1 - Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution

AU - Dong, Zhen Zhen

AU - Lu, Lihua

AU - Wang, Wanhe

AU - Li, Guodong

AU - Kang, Tian Shu

AU - Han, Simon Q B

AU - Leung, Chung Hang

AU - Ma, Edmond Dik Lung

PY - 2017/7

Y1 - 2017/7

N2 - A cyclometalated iridium(III) complex has been synthesized as a novel luminescent probe for G-quadruplex (G4) DNA in aqueous solution, and a G4-based assay was employed for the detection of nicking endonuclease activity using Nb.BsmI as a model enzyme. In the assay, an initial hairpin DNA (5′- TG4AG3TG4AG3TG4A2G3A2TGCTA3TAGCAT2C3T2C4AC-3′) sequence containing the Nb.BsmI recognition site in its stem region and a partially caged G-rich DNA sequence at its 5′-terminus could be cleaved by Nb.BsmI, which liberates the G-rich DNA sequence and enhances the luminescence of complex 1. This assay showed a linear relation between luminescent signal and Nb.BsmI concentration over the range of 0–10 U/mL (R2 = 0.9937) with a detection limit for Nb.BsmI of 0.042 U/mL. Furthermore, this assay showed high selectivity for Nb.BsmI over other nicking endonucleases, and functioned effectively in the presence of cell lysates.

AB - A cyclometalated iridium(III) complex has been synthesized as a novel luminescent probe for G-quadruplex (G4) DNA in aqueous solution, and a G4-based assay was employed for the detection of nicking endonuclease activity using Nb.BsmI as a model enzyme. In the assay, an initial hairpin DNA (5′- TG4AG3TG4AG3TG4A2G3A2TGCTA3TAGCAT2C3T2C4AC-3′) sequence containing the Nb.BsmI recognition site in its stem region and a partially caged G-rich DNA sequence at its 5′-terminus could be cleaved by Nb.BsmI, which liberates the G-rich DNA sequence and enhances the luminescence of complex 1. This assay showed a linear relation between luminescent signal and Nb.BsmI concentration over the range of 0–10 U/mL (R2 = 0.9937) with a detection limit for Nb.BsmI of 0.042 U/mL. Furthermore, this assay showed high selectivity for Nb.BsmI over other nicking endonucleases, and functioned effectively in the presence of cell lysates.

KW - G-quadruplex

KW - Iridium(III) complex

KW - Luminescence

KW - Nicking endonuclease

KW - Probe

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U2 - 10.1016/j.snb.2017.02.156

DO - 10.1016/j.snb.2017.02.156

M3 - Journal article

AN - SCOPUS:85014396112

SN - 0925-4005

VL - 246

SP - 826

EP - 832

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution

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