Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution

Zhen Zhen Dong, Lihua Lu, Wanhe Wang, Guodong Li, Tian Shu Kang, Simon Q B HAN, Chung Hang Leung*, Edmond Dik Lung MA

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A cyclometalated iridium(III) complex has been synthesized as a novel luminescent probe for G-quadruplex (G4) DNA in aqueous solution, and a G4-based assay was employed for the detection of nicking endonuclease activity using Nb.BsmI as a model enzyme. In the assay, an initial hairpin DNA (5′- TG4AG3TG4AG3TG4A2G3A2TGCTA3TAGCAT2C3T2C4AC-3′) sequence containing the Nb.BsmI recognition site in its stem region and a partially caged G-rich DNA sequence at its 5′-terminus could be cleaved by Nb.BsmI, which liberates the G-rich DNA sequence and enhances the luminescence of complex 1. This assay showed a linear relation between luminescent signal and Nb.BsmI concentration over the range of 0–10 U/mL (R2 = 0.9937) with a detection limit for Nb.BsmI of 0.042 U/mL. Furthermore, this assay showed high selectivity for Nb.BsmI over other nicking endonucleases, and functioned effectively in the presence of cell lysates.

Original languageEnglish
Pages (from-to)826-832
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume246
DOIs
Publication statusPublished - 2017

Scopus Subject Areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

User-Defined Keywords

  • G-quadruplex
  • Iridium(III) complex
  • Luminescence
  • Nicking endonuclease
  • Probe

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