TY - JOUR
T1 - Luminescent detection of human serum albumin in aqueous solution using a cyclometallated iridium(III) complex
AU - Lu, Lihua
AU - He, Hong Zhang
AU - Zhong, Hai Jing
AU - Liu, Li Juan
AU - Chan, Daniel Shiu Hin
AU - Leung, Chung Hang
AU - MA, Edmond Dik Lung
N1 - Funding Information:
This work is supported by Hong Kong Baptist University ( FRG2/12-13/021 and FRG2/13-14/008 ), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund ( HMRF/13121482 ), the Research Grants Council, University Grants Committee, Hong Kong ( HKBU/201811 , HKBU/204612 , and HKBU/201913 ), the French National Research Agency/Research Grants Council Joint Research Scheme ( A-HKBU201/12 ), the Science and Technology Development Fund, Macao SAR ( 103/2012/A3 ) and the University of Macau ( MYRG091(Y3-L2)-ICMS12-LCH, MYRG121(Y3-L2)-ICMS12-LCH, MRG007/LCH/2014/ICMS and MRG023/LCH/2013/ICMS ).
PY - 2014/10/1
Y1 - 2014/10/1
N2 - A novel cyclometalated iridium(III) complex has been developed as a luminescent probe for human serum albumin (HSA) detection in aqueous solution. In this system, the initial luminescence of the iridium(III) complex in the absence of human serum albumin is weak. However, the addition of HSA could greatly enhance the luminescence intensity of iridium(III) complex. The assay could detect down to 0.05 μM of HSA, and also exhibited good selectivity for HSA over bovine serum albumin and other biomolecules such as ATP, β-amyloid, peroxidase, thrombin, lysozyme, trypsin, hemoglobin, and the various nucleic acids. Furthermore, the application of the assay for the detection of HSA in diluted urine samples and for monitoring the conformational changes of HSA induced by exogenous protein unfolding agents was also demonstrated.
AB - A novel cyclometalated iridium(III) complex has been developed as a luminescent probe for human serum albumin (HSA) detection in aqueous solution. In this system, the initial luminescence of the iridium(III) complex in the absence of human serum albumin is weak. However, the addition of HSA could greatly enhance the luminescence intensity of iridium(III) complex. The assay could detect down to 0.05 μM of HSA, and also exhibited good selectivity for HSA over bovine serum albumin and other biomolecules such as ATP, β-amyloid, peroxidase, thrombin, lysozyme, trypsin, hemoglobin, and the various nucleic acids. Furthermore, the application of the assay for the detection of HSA in diluted urine samples and for monitoring the conformational changes of HSA induced by exogenous protein unfolding agents was also demonstrated.
KW - Human serum albumin
KW - Iridium(III) complex
KW - Luminescence
KW - Probe
UR - http://www.scopus.com/inward/record.url?scp=84901609554&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2014.04.100
DO - 10.1016/j.snb.2014.04.100
M3 - Article
AN - SCOPUS:84901609554
VL - 201
SP - 177
EP - 184
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
ER -