Luminescent detection of human serum albumin in aqueous solution using a cyclometallated iridium(III) complex

Lihua Lu, Hong Zhang He, Hai Jing Zhong, Li Juan Liu, Daniel Shiu Hin Chan, Chung Hang Leung, Edmond Dik Lung MA*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

A novel cyclometalated iridium(III) complex has been developed as a luminescent probe for human serum albumin (HSA) detection in aqueous solution. In this system, the initial luminescence of the iridium(III) complex in the absence of human serum albumin is weak. However, the addition of HSA could greatly enhance the luminescence intensity of iridium(III) complex. The assay could detect down to 0.05 μM of HSA, and also exhibited good selectivity for HSA over bovine serum albumin and other biomolecules such as ATP, β-amyloid, peroxidase, thrombin, lysozyme, trypsin, hemoglobin, and the various nucleic acids. Furthermore, the application of the assay for the detection of HSA in diluted urine samples and for monitoring the conformational changes of HSA induced by exogenous protein unfolding agents was also demonstrated.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume201
DOIs
Publication statusPublished - 1 Oct 2014

Scopus Subject Areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

User-Defined Keywords

  • Human serum albumin
  • Iridium(III) complex
  • Luminescence
  • Probe

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