Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex

Sheng Lin; Bingyong He; Chao Yang; Chung Hang Leung; Jean Louis Mergny; Edmond Dik Lung Ma

doi:10.1039/c5cc06655g

Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex

Sheng Lin, Bingyong He, Chao Yang, Chung Hang Leung^*, Jean Louis Mergny^*, Edmond Dik Lung Ma^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

50 Citations (Scopus)

Abstract

In this study, we synthesized a series of 9 luminescent iridium(iii) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii) complex 8 [Ir(pbtz)₂(dtbpy)]PF₆ (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.

Original language	English
Pages (from-to)	16033-16036
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	89
Early online date	14 Sept 2015
DOIs	https://doi.org/10.1039/c5cc06655g
Publication status	Published - 18 Nov 2015

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@article{01b2ee8316444ebe9201ec94083aaa22,

title = "Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex",

abstract = "In this study, we synthesized a series of 9 luminescent iridium(iii) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii) complex 8 [Ir(pbtz)2(dtbpy)]PF6 (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.",

author = "Sheng Lin and Bingyong He and Chao Yang and Leung, {Chung Hang} and Mergny, {Jean Louis} and Ma, {Edmond Dik Lung}",

note = "This work was supported by Hong Kong Baptist University (FRG2/14-15/004), the Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482 and HMRF/14130522), the Research Grants Council (HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12 – Oligoswitch), the Interdisciplinary Research Matching Scheme (RC-IRMS/14-15/06), the State Key Laboratory of Environmental and Biological Analysis Research Grant (SKLP-14-15-P001), the State Key Laboratory of Synthetic Chemistry, the Science and Technology Development Fund, Macao SAR (103/2012/A3 and 098/2014/A2) and the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH, MYRG2015-00137-ICMSQRCM and MRG023/LCH/2013/ICMS). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2015.",

year = "2015",

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doi = "10.1039/c5cc06655g",

language = "English",

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T1 - Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex

AU - Lin, Sheng

AU - He, Bingyong

AU - Yang, Chao

AU - Leung, Chung Hang

AU - Mergny, Jean Louis

AU - Ma, Edmond Dik Lung

N1 - This work was supported by Hong Kong Baptist University (FRG2/14-15/004), the Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482 and HMRF/14130522), the Research Grants Council (HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12 – Oligoswitch), the Interdisciplinary Research Matching Scheme (RC-IRMS/14-15/06), the State Key Laboratory of Environmental and Biological Analysis Research Grant (SKLP-14-15-P001), the State Key Laboratory of Synthetic Chemistry, the Science and Technology Development Fund, Macao SAR (103/2012/A3 and 098/2014/A2) and the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH, MYRG2015-00137-ICMSQRCM and MRG023/LCH/2013/ICMS). Publisher Copyright: © The Royal Society of Chemistry 2015.

PY - 2015/11/18

Y1 - 2015/11/18

N2 - In this study, we synthesized a series of 9 luminescent iridium(iii) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii) complex 8 [Ir(pbtz)2(dtbpy)]PF6 (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.

AB - In this study, we synthesized a series of 9 luminescent iridium(iii) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii) complex 8 [Ir(pbtz)2(dtbpy)]PF6 (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.

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U2 - 10.1039/c5cc06655g

DO - 10.1039/c5cc06655g

M3 - Journal article

C2 - 26399215

AN - SCOPUS:84946063326

SN - 1359-7345

VL - 51

SP - 16033

EP - 16036

JO - Chemical Communications

JF - Chemical Communications

IS - 89

ER -

Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex

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