Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex

Sheng Lin; Bingyong He; Chao Yang; Chung Hang Leung; Jean Louis Mergny; Edmond Dik Lung Ma

doi:10.1039/c5cc06655g

Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex

Sheng Lin, Bingyong He, Chao Yang, Chung Hang Leung^*, Jean Louis Mergny^*, Edmond Dik Lung Ma^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

48 Citations (Scopus)

Abstract

In this study, we synthesized a series of 9 luminescent iridium(iii) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii) complex 8 [Ir(pbtz)₂(dtbpy)]PF₆ (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.

Original language	English
Pages (from-to)	16033-16036
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	89
Early online date	14 Sept 2015
DOIs	https://doi.org/10.1039/c5cc06655g
Publication status	Published - 18 Nov 2015

Scopus Subject Areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex",

abstract = "In this study, we synthesized a series of 9 luminescent iridium(iii) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii) complex 8 [Ir(pbtz)2(dtbpy)]PF6 (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.",

author = "Sheng Lin and Bingyong He and Chao Yang and Leung, {Chung Hang} and Mergny, {Jean Louis} and Ma, {Edmond Dik Lung}",

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doi = "10.1039/c5cc06655g",

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T1 - Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex

AU - Lin, Sheng

AU - He, Bingyong

AU - Yang, Chao

AU - Leung, Chung Hang

AU - Mergny, Jean Louis

AU - Ma, Edmond Dik Lung

PY - 2015/11/18

Y1 - 2015/11/18

N2 - In this study, we synthesized a series of 9 luminescent iridium(iii) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii) complex 8 [Ir(pbtz)2(dtbpy)]PF6 (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.

AB - In this study, we synthesized a series of 9 luminescent iridium(iii) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii) complex 8 [Ir(pbtz)2(dtbpy)]PF6 (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.

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DO - 10.1039/c5cc06655g

M3 - Journal article

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AN - SCOPUS:84946063326

SN - 1359-7345

VL - 51

SP - 16033

EP - 16036

JO - Chemical Communications

JF - Chemical Communications

IS - 89

ER -

Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex

Abstract

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