@article{01b2ee8316444ebe9201ec94083aaa22,
title = "Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex",
abstract = "In this study, we synthesized a series of 9 luminescent iridium(iii) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii) complex 8 [Ir(pbtz)2(dtbpy)]PF6 (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.",
author = "Sheng Lin and Bingyong He and Chao Yang and Leung, {Chung Hang} and Mergny, {Jean Louis} and Ma, {Edmond Dik Lung}",
note = "This work was supported by Hong Kong Baptist University (FRG2/14-15/004), the Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482 and HMRF/14130522), the Research Grants Council (HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12 – Oligoswitch), the Interdisciplinary Research Matching Scheme (RC-IRMS/14-15/06), the State Key Laboratory of Environmental and Biological Analysis Research Grant (SKLP-14-15-P001), the State Key Laboratory of Synthetic Chemistry, the Science and Technology Development Fund, Macao SAR (103/2012/A3 and 098/2014/A2) and the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH, MYRG2015-00137-ICMSQRCM and MRG023/LCH/2013/ICMS). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2015.",
year = "2015",
month = nov,
day = "18",
doi = "10.1039/c5cc06655g",
language = "English",
volume = "51",
pages = "16033--16036",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "89",
}