Long-lived iridium(III) complexes as luminescent probes for the detection of periodate in living cells

Wanhe Wang; Lihua Lu; Ke Jia Wu; Jinshui Liu; Chung Hang Leung; Chun Yuen Wong; Dik Lung Ma

doi:10.1016/j.snb.2019.03.019

Long-lived iridium(III) complexes as luminescent probes for the detection of periodate in living cells

Wanhe Wang, Lihua Lu, Ke Jia Wu, Jinshui Liu, Chung Hang Leung, Chun Yuen Wong^*, Dik Lung Ma

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

25 Citations (Scopus)

Abstract

Periodate (IO₄⁻) is a widely used reagent in organic synthesis and for biomolecular modification. However, highly sensitive fluorescence detection methods for IO₄⁻ are lacking. In this work, we designed and synthesized iridium(III) complexes for the detection of IO₄⁻ by the incorporation of a methylthio group to serve as a luminescence trigger. The results showed that complex 1 could detect IO₄⁻ in a sensitive and selective manner with a detection limit of 0.077 μM, rendering it more sensitive than reported fluorescence methods. Meanwhile, its inherently long emission lifetime enables its luminescence response to IO₄⁻ to be detected even in the presence of organic dyes by the use of time-resolved emission spectroscopy (TRES). This probe was further demonstrated to detect IO₄⁻ in living cells. To our knowledge, complex 1 is first long-lived iridium(III)-based probe for the sensitive detection of IO₄⁻, and is also one of few existing imaging probes for IO₄⁻.

Original language	English
Pages (from-to)	392-398
Number of pages	7
Journal	Sensors and Actuators B: Chemical
Volume	288
DOIs	https://doi.org/10.1016/j.snb.2019.03.019
Publication status	Published - 1 Jun 2019

Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

User-Defined Keywords

Cell imaging
Iridium(III) complex
Long emission lifetime
Luminescence probe
Periodate
Time-resolved emission spectroscopy

Access to Document

10.1016/j.snb.2019.03.019

Cite this

@article{0c1013b51dd942bbbe6fd781a4fe03ec,

title = "Long-lived iridium(III) complexes as luminescent probes for the detection of periodate in living cells",

abstract = "Periodate (IO4−) is a widely used reagent in organic synthesis and for biomolecular modification. However, highly sensitive fluorescence detection methods for IO4− are lacking. In this work, we designed and synthesized iridium(III) complexes for the detection of IO4− by the incorporation of a methylthio group to serve as a luminescence trigger. The results showed that complex 1 could detect IO4− in a sensitive and selective manner with a detection limit of 0.077 μM, rendering it more sensitive than reported fluorescence methods. Meanwhile, its inherently long emission lifetime enables its luminescence response to IO4− to be detected even in the presence of organic dyes by the use of time-resolved emission spectroscopy (TRES). This probe was further demonstrated to detect IO4− in living cells. To our knowledge, complex 1 is first long-lived iridium(III)-based probe for the sensitive detection of IO4−, and is also one of few existing imaging probes for IO4−.",

keywords = "Cell imaging, Iridium(III) complex, Long emission lifetime, Luminescence probe, Periodate, Time-resolved emission spectroscopy",

author = "Wanhe Wang and Lihua Lu and Wu, {Ke Jia} and Jinshui Liu and Leung, {Chung Hang} and Wong, {Chun Yuen} and Ma, {Dik Lung}",

note = "Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/16-17/007 and FRG2/17-18/003 ), the Health and Medical Research Fund ( HMRF/14150561 ), the Research Grants Council ( HKBU/12301115 , CityU/11228316 , and CityU/11207117 ), the National Natural Science Foundation of China ( 21575121 and 21775131 ), the Hong Kong Baptist University Century Club Sponsorship Scheme 2018 , the Interdisciplinary Research Matching Scheme ( RC-IRMS/16-17/03 ), Interdisciplinary Research Clusters Matching Scheme ( RC-IRCs/17-18/03 ), Collaborative Research Fund ( C5026-16G ), SKLEBA and HKBU Strategic Development Fund ( SKLP_1718_P04 ), the Science and Technology Development Fund, Macao SAR ( 077/2016/A2 ), and the University of Macau ( MYRG2016-00151-ICMS-QRCM and MYRG2018-00187-ICMS ). ",

year = "2019",

month = jun,

day = "1",

doi = "10.1016/j.snb.2019.03.019",

language = "English",

volume = "288",

pages = "392--398",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier",

}

TY - JOUR

T1 - Long-lived iridium(III) complexes as luminescent probes for the detection of periodate in living cells

AU - Wang, Wanhe

AU - Lu, Lihua

AU - Wu, Ke Jia

AU - Liu, Jinshui

AU - Leung, Chung Hang

AU - Wong, Chun Yuen

AU - Ma, Dik Lung

N1 - Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/16-17/007 and FRG2/17-18/003 ), the Health and Medical Research Fund ( HMRF/14150561 ), the Research Grants Council ( HKBU/12301115 , CityU/11228316 , and CityU/11207117 ), the National Natural Science Foundation of China ( 21575121 and 21775131 ), the Hong Kong Baptist University Century Club Sponsorship Scheme 2018 , the Interdisciplinary Research Matching Scheme ( RC-IRMS/16-17/03 ), Interdisciplinary Research Clusters Matching Scheme ( RC-IRCs/17-18/03 ), Collaborative Research Fund ( C5026-16G ), SKLEBA and HKBU Strategic Development Fund ( SKLP_1718_P04 ), the Science and Technology Development Fund, Macao SAR ( 077/2016/A2 ), and the University of Macau ( MYRG2016-00151-ICMS-QRCM and MYRG2018-00187-ICMS ).

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Periodate (IO4−) is a widely used reagent in organic synthesis and for biomolecular modification. However, highly sensitive fluorescence detection methods for IO4− are lacking. In this work, we designed and synthesized iridium(III) complexes for the detection of IO4− by the incorporation of a methylthio group to serve as a luminescence trigger. The results showed that complex 1 could detect IO4− in a sensitive and selective manner with a detection limit of 0.077 μM, rendering it more sensitive than reported fluorescence methods. Meanwhile, its inherently long emission lifetime enables its luminescence response to IO4− to be detected even in the presence of organic dyes by the use of time-resolved emission spectroscopy (TRES). This probe was further demonstrated to detect IO4− in living cells. To our knowledge, complex 1 is first long-lived iridium(III)-based probe for the sensitive detection of IO4−, and is also one of few existing imaging probes for IO4−.

AB - Periodate (IO4−) is a widely used reagent in organic synthesis and for biomolecular modification. However, highly sensitive fluorescence detection methods for IO4− are lacking. In this work, we designed and synthesized iridium(III) complexes for the detection of IO4− by the incorporation of a methylthio group to serve as a luminescence trigger. The results showed that complex 1 could detect IO4− in a sensitive and selective manner with a detection limit of 0.077 μM, rendering it more sensitive than reported fluorescence methods. Meanwhile, its inherently long emission lifetime enables its luminescence response to IO4− to be detected even in the presence of organic dyes by the use of time-resolved emission spectroscopy (TRES). This probe was further demonstrated to detect IO4− in living cells. To our knowledge, complex 1 is first long-lived iridium(III)-based probe for the sensitive detection of IO4−, and is also one of few existing imaging probes for IO4−.

KW - Cell imaging

KW - Iridium(III) complex

KW - Long emission lifetime

KW - Luminescence probe

KW - Periodate

KW - Time-resolved emission spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85062693347&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2019.03.019

DO - 10.1016/j.snb.2019.03.019

M3 - Journal article

AN - SCOPUS:85062693347

SN - 0925-4005

VL - 288

SP - 392

EP - 398

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

Long-lived iridium(III) complexes as luminescent probes for the detection of periodate in living cells

Abstract

Scopus Subject Areas

User-Defined Keywords

Access to Document

Other files and links

Fingerprint

Cite this