Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling

Wei Zheng, Guo Liang Shen, Ke Yang Xu, Qiao Qiao Yin, Tian Chen Hui, Zhe Wen Zhou, Cheng An Xu, Shou Hao Wang, Wen Hao Wu, Ling Fei Shi, Hong Ying Pan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND 

Liver cancer is one of the most highly malignant cancers, characterized by easy metastasis and chemoradiotherapy resistance. Emerging evidence indicates that long noncoding RNAs (LncRNAs), including Lnc524369, are highly involved in the initiation, progression, radioresistance, and chemoresistance of hepatocellular carcinoma (HCC). However, the function of Lnc524369 remains unclear. 

AIM 

To explore the function of Lnc524369 in HCC. 

METHODS 

To investigate the effect of Lnc524369, tissue from 41 HCC patients were analyzed using CCK8, migration, and invasion assays. Lnc524369 and YWHAZ (also named 14-3-3ζ) mRNA were detected by qPCR, and YWHAZ and RAF1 proteins were detected by western blot in liver cancer cell lines and human HCC tissues. The Cancer Cell Line Encyclopedia (CCLE) databases, STRING database, Human Protein Atlas database, and the TCGA database were used for bioinformatic analysis. 

RESULTS 

Lnc524369 was significantly upregulated in the nucleus of liver cancer cells and human HCC tissues. Overexpression of Lnc524369 was associated with the proliferation, migration, and invasion of liver cancer cells. YWHAZ and RAF1 proteins and YWHAZ mRNA were overexpressed in liver cancer, which could be attenuated by overexpression of Lnc524369. Lnc524369 and its downstream target YWHAZ and RAF1 proteins were negatively associated with overall survival time. 

CONCLUSION 

Lnc524369 might be a promising target of HCC as it can enhance liver cancer progression and decrease the overall survival time of HCC by activating the YWHAZ/RAF1 pathway.

Original languageEnglish
Pages (from-to)253-264
Number of pages12
JournalWorld Journal of Gastrointestinal Oncology
Volume14
Issue number1
DOIs
Publication statusPublished - 15 Jan 2022

Scopus Subject Areas

  • Oncology
  • Gastroenterology

User-Defined Keywords

  • Hepatocellular carcinoma
  • Lnc524369
  • Long noncoding RNAs
  • RAF1
  • YWHAZ

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