Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling

Wei Zheng; Guo Liang Shen; Ke Yang  Xu; Qiao Qiao Yin; Tian Chen Hui; Zhe Wen Zhou; Cheng An Xu; Shou Hao Wang; Wen Hao  Wu; Ling Fei Shi; Hong Ying Pan

doi:10.4251/wjgo.v14.i1.253

Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling

Wei Zheng, Guo Liang Shen, Ke Yang Xu, Qiao Qiao Yin, Tian Chen Hui, Zhe Wen Zhou, Cheng An Xu, Shou Hao Wang, Wen Hao Wu, Ling Fei Shi, Hong Ying Pan^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

1 Citation (Scopus)

Abstract

BACKGROUND: Liver cancer is one of the most highly malignant cancers, characterized by easy metastasis and chemoradiotherapy resistance. Emerging evidence indicates that long noncoding RNAs (LncRNAs), including Lnc524369, are highly involved in the initiation, progression, radioresistance, and chemoresistance of hepatocellular carcinoma (HCC). However, the function of Lnc524369 remains unclear.

AIM: To explore the function of Lnc524369 in HCC.

METHODS: To investigate the effect of Lnc524369, tissue from 41 HCC patients were analyzed using CCK8, migration, and invasion assays. Lnc524369 and YWHAZ (also named 14-3-3ζ) mRNA were detected by qPCR, and YWHAZ and RAF1 proteins were detected by western blot in liver cancer cell lines and human HCC tissues. The Cancer Cell Line Encyclopedia (CCLE) databases, STRING database, Human Protein Atlas database, and the TCGA database were used for bioinformatic analysis.

RESULTS: Lnc524369 was significantly upregulated in the nucleus of liver cancer cells and human HCC tissues. Overexpression of Lnc524369 was associated with the proliferation, migration, and invasion of liver cancer cells. YWHAZ and RAF1 proteins and YWHAZ mRNA were overexpressed in liver cancer, which could be attenuated by overexpression of Lnc524369. Lnc524369 and its downstream target YWHAZ and RAF1 proteins were negatively associated with overall survival time.

CONCLUSION: Lnc524369 might be a promising target of HCC as it can enhance liver cancer progression and decrease the overall survival time of HCC by activating the YWHAZ/RAF1 pathway.

Original language	English
Pages (from-to)	253-264
Number of pages	12
Journal	World Journal of Gastrointestinal Oncology
Volume	14
Issue number	1
DOIs	https://doi.org/10.4251/wjgo.v14.i1.253
Publication status	Published - 15 Jan 2022

Scopus Subject Areas

Oncology
Gastroenterology

User-Defined Keywords

Hepatocellular carcinoma
Lnc524369
Long noncoding RNAs
RAF1
YWHAZ

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4251/wjgo.v14.i1.253

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@article{d8c202adcb184248b7d4abcc83d05b9d,

title = "Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling",

abstract = "BACKGROUND: Liver cancer is one of the most highly malignant cancers, characterized by easy metastasis and chemoradiotherapy resistance. Emerging evidence indicates that long noncoding RNAs (LncRNAs), including Lnc524369, are highly involved in the initiation, progression, radioresistance, and chemoresistance of hepatocellular carcinoma (HCC). However, the function of Lnc524369 remains unclear. AIM: To explore the function of Lnc524369 in HCC. METHODS: To investigate the effect of Lnc524369, tissue from 41 HCC patients were analyzed using CCK8, migration, and invasion assays. Lnc524369 and YWHAZ (also named 14-3-3ζ) mRNA were detected by qPCR, and YWHAZ and RAF1 proteins were detected by western blot in liver cancer cell lines and human HCC tissues. The Cancer Cell Line Encyclopedia (CCLE) databases, STRING database, Human Protein Atlas database, and the TCGA database were used for bioinformatic analysis. RESULTS: Lnc524369 was significantly upregulated in the nucleus of liver cancer cells and human HCC tissues. Overexpression of Lnc524369 was associated with the proliferation, migration, and invasion of liver cancer cells. YWHAZ and RAF1 proteins and YWHAZ mRNA were overexpressed in liver cancer, which could be attenuated by overexpression of Lnc524369. Lnc524369 and its downstream target YWHAZ and RAF1 proteins were negatively associated with overall survival time. CONCLUSION: Lnc524369 might be a promising target of HCC as it can enhance liver cancer progression and decrease the overall survival time of HCC by activating the YWHAZ/RAF1 pathway.",

keywords = "Hepatocellular carcinoma, Lnc524369, Long noncoding RNAs, RAF1, YWHAZ",

author = "Wei Zheng and Shen, {Guo Liang} and Xu, {Ke Yang} and Yin, {Qiao Qiao} and Hui, {Tian Chen} and Zhou, {Zhe Wen} and Xu, {Cheng An} and Wang, {Shou Hao} and Wu, {Wen Hao} and Shi, {Ling Fei} and Pan, {Hong Ying}",

note = "Funding Information: Supported by the Medical and Health Science and Technology Project of Zhejiang Province (No. 2021KY043) and Chinese Foundation for Hepatitis Prevention and Control-TianQing Liver Disease Research Fund Subject (No. TQGB2020168). Publisher Copyright: {\textcopyright} 2022. The Author(s). Published by Baishideng Publishing Group Inc. All Rights Reserved.",

year = "2022",

month = jan,

day = "15",

doi = "10.4251/wjgo.v14.i1.253",

language = "English",

volume = "14",

pages = "253--264",

journal = "World Journal of Gastrointestinal Oncology",

issn = "1948-5204",

publisher = "Baishideng Publishing Group",

number = "1",

}

TY - JOUR

T1 - Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling

AU - Zheng, Wei

AU - Shen, Guo Liang

AU - Xu, Ke Yang

AU - Yin, Qiao Qiao

AU - Hui, Tian Chen

AU - Zhou, Zhe Wen

AU - Xu, Cheng An

AU - Wang, Shou Hao

AU - Wu, Wen Hao

AU - Shi, Ling Fei

AU - Pan, Hong Ying

N1 - Funding Information: Supported by the Medical and Health Science and Technology Project of Zhejiang Province (No. 2021KY043) and Chinese Foundation for Hepatitis Prevention and Control-TianQing Liver Disease Research Fund Subject (No. TQGB2020168). Publisher Copyright: © 2022. The Author(s). Published by Baishideng Publishing Group Inc. All Rights Reserved.

PY - 2022/1/15

Y1 - 2022/1/15

N2 - BACKGROUND: Liver cancer is one of the most highly malignant cancers, characterized by easy metastasis and chemoradiotherapy resistance. Emerging evidence indicates that long noncoding RNAs (LncRNAs), including Lnc524369, are highly involved in the initiation, progression, radioresistance, and chemoresistance of hepatocellular carcinoma (HCC). However, the function of Lnc524369 remains unclear. AIM: To explore the function of Lnc524369 in HCC. METHODS: To investigate the effect of Lnc524369, tissue from 41 HCC patients were analyzed using CCK8, migration, and invasion assays. Lnc524369 and YWHAZ (also named 14-3-3ζ) mRNA were detected by qPCR, and YWHAZ and RAF1 proteins were detected by western blot in liver cancer cell lines and human HCC tissues. The Cancer Cell Line Encyclopedia (CCLE) databases, STRING database, Human Protein Atlas database, and the TCGA database were used for bioinformatic analysis. RESULTS: Lnc524369 was significantly upregulated in the nucleus of liver cancer cells and human HCC tissues. Overexpression of Lnc524369 was associated with the proliferation, migration, and invasion of liver cancer cells. YWHAZ and RAF1 proteins and YWHAZ mRNA were overexpressed in liver cancer, which could be attenuated by overexpression of Lnc524369. Lnc524369 and its downstream target YWHAZ and RAF1 proteins were negatively associated with overall survival time. CONCLUSION: Lnc524369 might be a promising target of HCC as it can enhance liver cancer progression and decrease the overall survival time of HCC by activating the YWHAZ/RAF1 pathway.

AB - BACKGROUND: Liver cancer is one of the most highly malignant cancers, characterized by easy metastasis and chemoradiotherapy resistance. Emerging evidence indicates that long noncoding RNAs (LncRNAs), including Lnc524369, are highly involved in the initiation, progression, radioresistance, and chemoresistance of hepatocellular carcinoma (HCC). However, the function of Lnc524369 remains unclear. AIM: To explore the function of Lnc524369 in HCC. METHODS: To investigate the effect of Lnc524369, tissue from 41 HCC patients were analyzed using CCK8, migration, and invasion assays. Lnc524369 and YWHAZ (also named 14-3-3ζ) mRNA were detected by qPCR, and YWHAZ and RAF1 proteins were detected by western blot in liver cancer cell lines and human HCC tissues. The Cancer Cell Line Encyclopedia (CCLE) databases, STRING database, Human Protein Atlas database, and the TCGA database were used for bioinformatic analysis. RESULTS: Lnc524369 was significantly upregulated in the nucleus of liver cancer cells and human HCC tissues. Overexpression of Lnc524369 was associated with the proliferation, migration, and invasion of liver cancer cells. YWHAZ and RAF1 proteins and YWHAZ mRNA were overexpressed in liver cancer, which could be attenuated by overexpression of Lnc524369. Lnc524369 and its downstream target YWHAZ and RAF1 proteins were negatively associated with overall survival time. CONCLUSION: Lnc524369 might be a promising target of HCC as it can enhance liver cancer progression and decrease the overall survival time of HCC by activating the YWHAZ/RAF1 pathway.

KW - Hepatocellular carcinoma

KW - Lnc524369

KW - Long noncoding RNAs

KW - RAF1

KW - YWHAZ

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U2 - 10.4251/wjgo.v14.i1.253

DO - 10.4251/wjgo.v14.i1.253

M3 - Journal article

AN - SCOPUS:85123255249

SN - 1948-5204

VL - 14

SP - 253

EP - 264

JO - World Journal of Gastrointestinal Oncology

JF - World Journal of Gastrointestinal Oncology

IS - 1

ER -

Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling

Abstract

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