TY - JOUR
T1 - Liver Protection of a Low-Polarity Fraction from Ficus pandurata Hance, Prepared by Supercritical CO2 Fluid Extraction, on CCl4-Induced Acute Liver Injury in Mice via Inhibiting Apoptosis and Ferroptosis Mediated by Strengthened Antioxidation
AU - Dai, Weibo
AU - Pang, Xiaoyan
AU - Peng, Weiwen
AU - Zhan, Xinyi
AU - Chen, Chang
AU - Zhao, Wenchang
AU - Zeng, Congyan
AU - Mei, Quanxi
AU - Chen, Qilei
AU - Kuang, Weihong
AU - Gou, Zhanping
AU - Hu, Xianjing
N1 - The authors acknowledge the financial support of the National Natural Science Foundation of China (81503303, 81973548, and 81473401), Discipline Construction Project of Guangdong Medical University (4SG22009G and 4SG21009G), Guangdong Innovation Team Project for Regular Institutions of Higher Learning (2022KCXTD011), Guangdong Medical University Research Foundation (4SG22242G), Traditional Chinese Medicine Bureau of Guangdong Province, China (20213011), Project of Administration of Traditional Chinese Medicine of Guangdong Province of China (20182168), and Special Fund for Science and Technology Development of Zhanjiang (2022A01003).
Publisher Copyright:
© 2023 by the authors.
PY - 2023/3/1
Y1 - 2023/3/1
N2 - Ficus pandurata Hance (FPH) is a Chinese herbal medicine widely used for health care. This study was designed to investigate the alleviation efficacy of the low-polarity ingredients of FPH (FPHLP), prepared by supercritical CO2 fluid extraction technology, against CCl4-induced acute liver injury (ALI) in mice and uncover its underlying mechanism. The results showed that FPHLP had a good antioxidative effect determined by the DPPH free radical scavenging activity test and T-AOC assay. The in vivo study showed that FPHLP dose-dependently protected against liver damage via detection of ALT, AST, and LDH levels and changes in liver histopathology. The antioxidative stress properties of FPHLP suppressed ALI by increasing levels of GSH, Nrf2, HO-1, and Trx-1 and reducing levels of ROS and MDA and the expression of Keap1. FPHLP significantly reduced the level of Fe2+ and expression of TfR1, xCT/SLC7A11, and Bcl2, while increasing the expression of GPX4, FTH1, cleaved PARP, Bax, and cleaved caspase 3. The results demonstrated that FPHLP protected mouse liver from injury induced by CCl4 via suppression of apoptosis and ferroptosis. This study suggests that FPHLP can be used for liver damage protection in humans, which strongly supports its traditional use as a herbal medicine.
AB - Ficus pandurata Hance (FPH) is a Chinese herbal medicine widely used for health care. This study was designed to investigate the alleviation efficacy of the low-polarity ingredients of FPH (FPHLP), prepared by supercritical CO2 fluid extraction technology, against CCl4-induced acute liver injury (ALI) in mice and uncover its underlying mechanism. The results showed that FPHLP had a good antioxidative effect determined by the DPPH free radical scavenging activity test and T-AOC assay. The in vivo study showed that FPHLP dose-dependently protected against liver damage via detection of ALT, AST, and LDH levels and changes in liver histopathology. The antioxidative stress properties of FPHLP suppressed ALI by increasing levels of GSH, Nrf2, HO-1, and Trx-1 and reducing levels of ROS and MDA and the expression of Keap1. FPHLP significantly reduced the level of Fe2+ and expression of TfR1, xCT/SLC7A11, and Bcl2, while increasing the expression of GPX4, FTH1, cleaved PARP, Bax, and cleaved caspase 3. The results demonstrated that FPHLP protected mouse liver from injury induced by CCl4 via suppression of apoptosis and ferroptosis. This study suggests that FPHLP can be used for liver damage protection in humans, which strongly supports its traditional use as a herbal medicine.
KW - antioxidation
KW - apoptosis
KW - ferroptosis
KW - Ficus pandurata Hance
KW - liver injury
KW - supercritical CO fluid extraction
UR - http://www.scopus.com/inward/record.url?scp=85150203429&partnerID=8YFLogxK
U2 - 10.3390/molecules28052078
DO - 10.3390/molecules28052078
M3 - Journal article
C2 - 36903326
AN - SCOPUS:85150203429
SN - 1420-3049
VL - 28
JO - Molecules
JF - Molecules
IS - 5
M1 - 2078
ER -
