Lipidomics identification of metabolic biomarkers in chemically induced hypertriglyceridemic mice

Hiu Yee KWAN, Yong Mei Hu, Chi Leung Chan, Hui Hui Cao, Chi Yan Cheng, Si Yuan Pan, Anfernee K W TSE, Yiu Cheong Wu, Zhiling YU*, David W F FONG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

In this study, we aim to identify the potential biomarkers in hTG pathogenesis in schisandrin B-induced hTG mouse model. To investigate whether these identified biomarkers are only specific to schisandrin B-induced hTG mouse model, we also measured these biomarkers in a high fat diet (HFD)-induced hTG mouse model. We employed a LC/MS/MS-based lipidomic approach for the study. Mouse liver and serum metabolites were separated by reversed phase liquid chromatography. Metabolite candidates were identified by matching with marker retention times, isotope distribution patterns, and high-resolution MS/MS fragmentation patterns. Subsequently, target candidates were quantified by quantitative MS. In the schisandrin B-induced hTG mice, we found that the plasma fatty acids, diglyceroids, and phospholipids were significantly increased. Palmitic acid and stearic acid were increased in the plasma; oleic acid, linoleic acid, linolenic acid, arachidonic acid, and docosahexaenoic acid were increased in both the plasma and the liver. Acetyl-CoA, malonyl-CoA, and succinyl-CoA were increased only in the liver. The changes in levels of these identified markers were also observed in HFD-induced hTG mouse model. The consistent results obtained from both hTG models not only suggest novel biomarkers in hTG pathogenesis, but they also provide insight into the underlying mechanism of the schisandrin B-induced hTG.

Original languageEnglish
Pages (from-to)1387-1398
Number of pages12
JournalJournal of Proteome Research
Volume12
Issue number3
DOIs
Publication statusPublished - Mar 2013

Scopus Subject Areas

  • Biochemistry
  • Chemistry(all)

User-Defined Keywords

  • high-resolution MS/MS fragmentation
  • malonyl CoA
  • quantification
  • reversed phase liquid chromatography (RPLC)

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