Lipid accumulation potential of oleaginous yeasts: A comparative evaluation using food waste leachate as a substrate

Johnravindar Davidraj; Obulisamy Parthiba Karthikeyan; Ammaiyappan Selvam; Kumarasamy Murugesan; Jonathan W.C. Wong

doi:10.1016/j.biortech.2017.06.151

Lipid accumulation potential of oleaginous yeasts: A comparative evaluation using food waste leachate as a substrate

Johnravindar Davidraj
, Obulisamy Parthiba Karthikeyan
, Ammaiyappan Selvam
, Kumarasamy Murugesan
, Jonathan W.C. Wong^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

63 Citations (Scopus)

71 Downloads (Pure)

Abstract

In present study, the efficiency of three oleaginous yeasts i.e., Yarrowia lipolytica, Rhodotorula glutinis and Cryptococcus curvatus were compared for their lipid assimilation capacities using three different FW-leachates as a medium. The FW-leachates were collected from dry anaerobic digesters and diluted to achieve carbohydrate content of 25 g L⁻¹ prior to yeast inoculations. Around 5% of yeast cultures were individually mixed in three different FW-leachate mediums and incubated under 30 °C and 150 rpm agitation for 6 days. The Y. lipolytica produced high biomass with lipid contents of 49.0 ± 2% on dry weight basis. Whereas, the acetic acid concentration of >6 g L⁻¹ inhibited the growth of R. glutinis. The study observed that the selection of appropriate FW-leachate composition is highly important for biolipid accumulation by oleaginous yeasts.

Original language	English
Pages (from-to)	221-228
Number of pages	8
Journal	Bioresource Technology
Volume	248
DOIs	https://doi.org/10.1016/j.biortech.2017.06.151
Publication status	Published - Jan 2018

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SDG 7 Affordable and Clean Energy

User-Defined Keywords

Biolipids
Food waste
Oleaginous yeast
Pre-treatment
Volatile fatty acids

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10.1016/j.biortech.2017.06.151

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@article{4c5fb614b1f149728c5e3f229ebcf989,

title = "Lipid accumulation potential of oleaginous yeasts: A comparative evaluation using food waste leachate as a substrate",

abstract = "In present study, the efficiency of three oleaginous yeasts i.e., Yarrowia lipolytica, Rhodotorula glutinis and Cryptococcus curvatus were compared for their lipid assimilation capacities using three different FW-leachates as a medium. The FW-leachates were collected from dry anaerobic digesters and diluted to achieve carbohydrate content of 25 g L−1 prior to yeast inoculations. Around 5\% of yeast cultures were individually mixed in three different FW-leachate mediums and incubated under 30 °C and 150 rpm agitation for 6 days. The Y. lipolytica produced high biomass with lipid contents of 49.0 ± 2\% on dry weight basis. Whereas, the acetic acid concentration of >6 g L−1 inhibited the growth of R. glutinis. The study observed that the selection of appropriate FW-leachate composition is highly important for biolipid accumulation by oleaginous yeasts.",

keywords = "Biolipids, Food waste, Oleaginous yeast, Pre-treatment, Volatile fatty acids",

author = "Johnravindar Davidraj and Karthikeyan, \{Obulisamy Parthiba\} and Ammaiyappan Selvam and Kumarasamy Murugesan and Wong, \{Jonathan W.C.\}",

note = "Funding information: The authors would like to thank the Research Grant Council, Hong Kong Special Administrative Region for the funding support provided under Collaborative Research Fund (CRF; Grant No: C6033-14G). Publisher copyright: {\textcopyright} 2017 Elsevier Ltd.",

year = "2018",

month = jan,

doi = "10.1016/j.biortech.2017.06.151",

language = "English",

volume = "248",

pages = "221--228",

journal = "Bioresource Technology",

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publisher = "Elsevier Ltd.",

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TY - JOUR

T1 - Lipid accumulation potential of oleaginous yeasts

T2 - A comparative evaluation using food waste leachate as a substrate

AU - Davidraj, Johnravindar

AU - Karthikeyan, Obulisamy Parthiba

AU - Selvam, Ammaiyappan

AU - Murugesan, Kumarasamy

AU - Wong, Jonathan W.C.

N1 - Funding information: The authors would like to thank the Research Grant Council, Hong Kong Special Administrative Region for the funding support provided under Collaborative Research Fund (CRF; Grant No: C6033-14G). Publisher copyright: © 2017 Elsevier Ltd.

PY - 2018/1

Y1 - 2018/1

N2 - In present study, the efficiency of three oleaginous yeasts i.e., Yarrowia lipolytica, Rhodotorula glutinis and Cryptococcus curvatus were compared for their lipid assimilation capacities using three different FW-leachates as a medium. The FW-leachates were collected from dry anaerobic digesters and diluted to achieve carbohydrate content of 25 g L−1 prior to yeast inoculations. Around 5% of yeast cultures were individually mixed in three different FW-leachate mediums and incubated under 30 °C and 150 rpm agitation for 6 days. The Y. lipolytica produced high biomass with lipid contents of 49.0 ± 2% on dry weight basis. Whereas, the acetic acid concentration of >6 g L−1 inhibited the growth of R. glutinis. The study observed that the selection of appropriate FW-leachate composition is highly important for biolipid accumulation by oleaginous yeasts.

AB - In present study, the efficiency of three oleaginous yeasts i.e., Yarrowia lipolytica, Rhodotorula glutinis and Cryptococcus curvatus were compared for their lipid assimilation capacities using three different FW-leachates as a medium. The FW-leachates were collected from dry anaerobic digesters and diluted to achieve carbohydrate content of 25 g L−1 prior to yeast inoculations. Around 5% of yeast cultures were individually mixed in three different FW-leachate mediums and incubated under 30 °C and 150 rpm agitation for 6 days. The Y. lipolytica produced high biomass with lipid contents of 49.0 ± 2% on dry weight basis. Whereas, the acetic acid concentration of >6 g L−1 inhibited the growth of R. glutinis. The study observed that the selection of appropriate FW-leachate composition is highly important for biolipid accumulation by oleaginous yeasts.

KW - Biolipids

KW - Food waste

KW - Oleaginous yeast

KW - Pre-treatment

KW - Volatile fatty acids

UR - https://www.scopus.com/pages/publications/85025147407

U2 - 10.1016/j.biortech.2017.06.151

DO - 10.1016/j.biortech.2017.06.151

M3 - Journal article

C2 - 28736146

AN - SCOPUS:85025147407

SN - 0960-8524

VL - 248

SP - 221

EP - 228

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Lipid accumulation potential of oleaginous yeasts: A comparative evaluation using food waste leachate as a substrate

Abstract

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