Leocarpinolide B attenuates LPS-induced inflammation on RAW264.7 macrophages by mediating NF-κB and Nrf2 pathways

Ke Gang Linghu, Qiu Shuo Ma, Guan Ding Zhao, Wei Xiong, Ligen Lin, Qing Wen Zhang, Zhaoxiang BIAN, Yitao Wang, Hua Yu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Macrophages-mediated inflammation is involved in the regulation of rheumatoid arthritis (RA). Sigesbeckiae Herba (SH) has been traditionally used for rheumatism. However, the bioactive ingredients of SH are still unclear. Recently, we isolated a compound (Leocarpinolide B, LB) from SH and identified its potent anti-inflammatory and antioxidant effects on RAW264.7 macrophages for the first time. LB effectively inhibited excessive production of nitric oxide (NO), prostaglandin E2 (PGE2), cytokines (IL-6, TNF-α and MCP-1), and the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthases (iNOS) in lipopolysaccharide (LPS)-induced RAW264.7 cells. LB blocked the degradation of inhibitor of kappa B (IκBα) and translocation of nuclear factor kappa B (NF-κB) p65. Additionally, LB reduced the intracellular reactive oxygen species, and increased the expression of heme oxygenase-1 (HO-1) and the translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) in the presence or absence of LPS. The results suggested that LB might be one of the bioactive components of SH, and be potential for the treatment of RA and valuable to be further investigated.

Original languageEnglish
Article number172854
JournalEuropean Journal of Pharmacology
Volume868
DOIs
Publication statusPublished - 5 Feb 2020

Scopus Subject Areas

  • Pharmacology

User-Defined Keywords

  • Leocarpinolide B
  • Nuclear factor erythroid 2-related factor 2 (Nrf2)
  • Nuclear transcription factor-kappa B (NF-κB)
  • Rheumatoid arthritis (RA)
  • Sigesbeckiae herba (SH)

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