LC-MS/MS in the routine clinical laboratory: Has its time come?

Kelvin S Y LEUNG; Bonnie Mei Wah Fong

doi:10.1007/s00216-013-7542-5

LC-MS/MS in the routine clinical laboratory: Has its time come?

Kelvin S Y LEUNG^*, Bonnie Mei Wah Fong

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Review article › peer-review

64 Citations (Scopus)

Abstract

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly used in routine clinical laboratories during the last two decades. The high specificity, sensitivity, and multi-analyte potential make it an ideal alternative to immunoassays or conventional high-performance liquid chromatography (HPLC). It also provides higher throughput than gas chromatography-mass spectrometry (GC-MS). LC-MS/MS also offers higher flexibility than immunoassays because LC-MS/MS assays are typically developed in-house. In addition, abundant information can be obtained from a single LC-MS/MS run which can produce a large amount of quantitative or qualitative data. In this review, typical LC-MS/MS clinical applications are presented, personal experiences are shared, and strengths and weakness are discussed. It is foreseeable that LC-MS/MS will become a key instrument in routine clinical laboratories.

Original language	English
Pages (from-to)	2289-2301
Number of pages	13
Journal	Analytical and Bioanalytical Chemistry
Volume	406
Issue number	9-10
DOIs	https://doi.org/10.1007/s00216-013-7542-5
Publication status	Published - Apr 2014

Scopus Subject Areas

Analytical Chemistry
Biochemistry

User-Defined Keywords

LC-MS/MS
Routine clinical applications
Strengths
Weakness

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10.1007/s00216-013-7542-5

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abstract = "Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly used in routine clinical laboratories during the last two decades. The high specificity, sensitivity, and multi-analyte potential make it an ideal alternative to immunoassays or conventional high-performance liquid chromatography (HPLC). It also provides higher throughput than gas chromatography-mass spectrometry (GC-MS). LC-MS/MS also offers higher flexibility than immunoassays because LC-MS/MS assays are typically developed in-house. In addition, abundant information can be obtained from a single LC-MS/MS run which can produce a large amount of quantitative or qualitative data. In this review, typical LC-MS/MS clinical applications are presented, personal experiences are shared, and strengths and weakness are discussed. It is foreseeable that LC-MS/MS will become a key instrument in routine clinical laboratories.",

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LC-MS/MS in the routine clinical laboratory: Has its time come?

Abstract

Scopus Subject Areas

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