LC-MS/MS in the routine clinical laboratory: Has its time come?

Kelvin S Y LEUNG*, Bonnie Mei Wah Fong

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

77 Citations (Scopus)


Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly used in routine clinical laboratories during the last two decades. The high specificity, sensitivity, and multi-analyte potential make it an ideal alternative to immunoassays or conventional high-performance liquid chromatography (HPLC). It also provides higher throughput than gas chromatography-mass spectrometry (GC-MS). LC-MS/MS also offers higher flexibility than immunoassays because LC-MS/MS assays are typically developed in-house. In addition, abundant information can be obtained from a single LC-MS/MS run which can produce a large amount of quantitative or qualitative data. In this review, typical LC-MS/MS clinical applications are presented, personal experiences are shared, and strengths and weakness are discussed. It is foreseeable that LC-MS/MS will become a key instrument in routine clinical laboratories.

Original languageEnglish
Pages (from-to)2289-2301
Number of pages13
JournalAnalytical and Bioanalytical Chemistry
Issue number9-10
Publication statusPublished - Apr 2014

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry

User-Defined Keywords

  • LC-MS/MS
  • Routine clinical applications
  • Strengths
  • Weakness


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