TY - JOUR
T1 - LC-MS/MS in the routine clinical laboratory
T2 - Has its time come?
AU - Leung, Kelvin Sze Yin
AU - Fong, Bonnie Mei Wah
N1 - Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2014/4
Y1 - 2014/4
N2 - Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly used in routine clinical laboratories during the last two decades. The high specificity, sensitivity, and multi-analyte potential make it an ideal alternative to immunoassays or conventional high-performance liquid chromatography (HPLC). It also provides higher throughput than gas chromatography-mass spectrometry (GC-MS). LC-MS/MS also offers higher flexibility than immunoassays because LC-MS/MS assays are typically developed in-house. In addition, abundant information can be obtained from a single LC-MS/MS run which can produce a large amount of quantitative or qualitative data. In this review, typical LC-MS/MS clinical applications are presented, personal experiences are shared, and strengths and weakness are discussed. It is foreseeable that LC-MS/MS will become a key instrument in routine clinical laboratories.
AB - Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly used in routine clinical laboratories during the last two decades. The high specificity, sensitivity, and multi-analyte potential make it an ideal alternative to immunoassays or conventional high-performance liquid chromatography (HPLC). It also provides higher throughput than gas chromatography-mass spectrometry (GC-MS). LC-MS/MS also offers higher flexibility than immunoassays because LC-MS/MS assays are typically developed in-house. In addition, abundant information can be obtained from a single LC-MS/MS run which can produce a large amount of quantitative or qualitative data. In this review, typical LC-MS/MS clinical applications are presented, personal experiences are shared, and strengths and weakness are discussed. It is foreseeable that LC-MS/MS will become a key instrument in routine clinical laboratories.
KW - LC-MS/MS
KW - Routine clinical applications
KW - Strengths
KW - Weakness
UR - http://www.scopus.com/inward/record.url?scp=84898035120&partnerID=8YFLogxK
U2 - 10.1007/s00216-013-7542-5
DO - 10.1007/s00216-013-7542-5
M3 - Review article
C2 - 24337187
AN - SCOPUS:84898035120
SN - 1618-2642
VL - 406
SP - 2289
EP - 2301
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 9-10
ER -