Layer-by-layer assembly of graphene, Au and poly(toluidine blue O) films sensor for evaluation of oxidative stress of tumor cells elicited by hydrogen peroxide

Hucheng Chang, Xuemei Wang*, Kwok Keung SHIU, Yanliang Zhu, Jianling Wang, Qiwei Li, Baoan Chen, Hui Jiang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)

Abstract

High level of oxidative stress is involved in formation of incipient tumor and carcinomatous cells. Here in this contribution we have explored a facile strategy to assess the oxidative stress elicited by hydrogen peroxide (H2O2) in cells with amperometric current-time technique in vitro. An electrochemical biosensor exhibiting high sensitivity and selectivity to H2O2 is fabricated by integration of graphene with gold nanoparticles and poly(toluidine blue O) films. The efflux of H2O2 from several representative tumor cells and normal cells on exposure to ascorbic acid could be detected by using the graphene-based nanocomposite films. The results indicate that tumor cells release much more H2O2 than do the normal cells. The novel sensor raises the possibility for clinical diagnostic application to evaluate the higher level of intracellular oxidative stress of tumor cells in comparison with normal cells.

Original languageEnglish
Pages (from-to)789-794
Number of pages6
JournalBiosensors and Bioelectronics
Volume41
Issue number1
DOIs
Publication statusPublished - 15 Mar 2013

Scopus Subject Areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

User-Defined Keywords

  • Electrochemical detection
  • Graphene nanocomposites
  • Layer-by-layer assembly
  • Oxidative stress
  • Tumor cells

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