Lanthanide-Based Peptide-Directed Visible/Near-Infrared Imaging and Inhibition of LMP1

Ho-Fai Chau, Yue Wu, Wan-Yiu Fok, Waygen Thor, William Chi-Shing Cho, Ping'an Ma, Jun Lin, Nai-Ki Mak, Jean-Claude G. Bunzli*, Lijun Jiang*, Nicholas J. Long*, Hong Lok Lung*, Ka-Leung Wong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A lanthanide-based peptide-directed bioprobe LnP19 (Ln = Eu or Yb) is designed as an impressive example of a small molecule-based dual-functional probe for the EBV oncoprotein LMP1. The peptide P19 (Pra-KAhx-K-LDLALK-FWLY-K-IVMSDKW-K-RrRK) is designed to selectively bind to LMP1 by mimicking its TM1 region during oligomerization in lipid rafts while signal transduction is significantly suppressed. Immunofluorescence imaging and Western blotting results reveal that P19 can effectively inactivate the oncogenic cellular pathway nuclear factor κB (NF-κB) and contribute to a selective cytotoxic effect on LMP1-positive cells. By conjugation with cyclen-based europium(III) and ytterbium(III) complexes, EuP19 and YbP19 were constructed to offer visible and near-infrared LMP1-targeted imaging and cancer monitoring. In addition to the ability to target and inhibit LMP1 and to selective inhibit LMP1-positive cells, selective growth inhibition toward the LMP1-positive tumor by LnP19 is also demonstrated.
Original languageEnglish
Pages (from-to)1034-1043
Number of pages10
JournalJournal of American Chemical Society AU
Volume1
Issue number7
DOIs
Publication statusPublished - 26 Jul 2021

User-Defined Keywords

  • LMP1-targeted imaging EBV
  • EBV-related cancer
  • lanthanide(III) luminescence
  • theranostic agent

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