Label-free luminescent switch-on probe for ochratoxin a detection using a G-quadruplex-selective iridium(III) complex

Lihua Lu; Modi Wang; Li Juan Liu; Chung Hang Leung; Dik Lung Ma

doi:10.1021/acsami.5b01702

Label-free luminescent switch-on probe for ochratoxin a detection using a G-quadruplex-selective iridium(III) complex

Lihua Lu
, Modi Wang
, Li Juan Liu
, Chung Hang Leung^*
, Dik Lung Ma^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

45 Citations (Scopus)

Abstract

A library of six luminescent Ir(III) complexes were synthesized and studied for their capacity to function as probes for G-quadruplex DNA. The novel Ir(III) complex 1 was discovered to be selective for G-quadruplex structures and was subsequently used for the construction of a label-free G-quadruplex-based ochratoxin A (OTA) sensing platform in aqueous solution. The assay exhibited linearity for OTA in the range of 0 to 60 nM (R² = 0.9933), and the limit of detection for OTA was 5 nM. Furthermore, this assay was highly selective for OTA over its structurally related analogues.

Original language	English
Pages (from-to)	8313-8318
Number of pages	6
Journal	ACS Applied Materials and Interfaces
Volume	7
Issue number	15
DOIs	https://doi.org/10.1021/acsami.5b01702
Publication status	Published - 22 Apr 2015

User-Defined Keywords

G-quadruplex
iridium(III) complex
label-free
luminescent
ochratoxin A

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10.1021/acsami.5b01702

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title = "Label-free luminescent switch-on probe for ochratoxin a detection using a G-quadruplex-selective iridium(III) complex",

abstract = "A library of six luminescent Ir(III) complexes were synthesized and studied for their capacity to function as probes for G-quadruplex DNA. The novel Ir(III) complex 1 was discovered to be selective for G-quadruplex structures and was subsequently used for the construction of a label-free G-quadruplex-based ochratoxin A (OTA) sensing platform in aqueous solution. The assay exhibited linearity for OTA in the range of 0 to 60 nM (R2 = 0.9933), and the limit of detection for OTA was 5 nM. Furthermore, this assay was highly selective for OTA over its structurally related analogues.",

keywords = "G-quadruplex, iridium(III) complex, label-free, luminescent, ochratoxin A",

author = "Lihua Lu and Modi Wang and Liu, \{Li Juan\} and Leung, \{Chung Hang\} and Ma, \{Dik Lung\}",

note = "Funding information: This work is supported by Hong Kong Baptist University (FRG2/13-14/008 and FRG2/14-15/004), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482 and HMRF/14130522), the Research Grants Council (HKBU/201811, HKBU/204612, and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), State Key Laboratory of Environmental and Biological Analysis Research Grant (SKLP-14-15-P001), the State Key Laboratory of Synthetic Chemistry, the Science and Technology Development Fund, Macao SAR (103/2012/A3), and the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH, MYRG2015-00137-ICMS-QRCM, and MRG023/LCH/2013/ICMS). Publisher copyright: {\textcopyright} 2015 American Chemical Society",

year = "2015",

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doi = "10.1021/acsami.5b01702",

language = "English",

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AU - Lu, Lihua

AU - Wang, Modi

AU - Liu, Li Juan

AU - Leung, Chung Hang

AU - Ma, Dik Lung

N1 - Funding information: This work is supported by Hong Kong Baptist University (FRG2/13-14/008 and FRG2/14-15/004), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482 and HMRF/14130522), the Research Grants Council (HKBU/201811, HKBU/204612, and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), State Key Laboratory of Environmental and Biological Analysis Research Grant (SKLP-14-15-P001), the State Key Laboratory of Synthetic Chemistry, the Science and Technology Development Fund, Macao SAR (103/2012/A3), and the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH, MYRG2015-00137-ICMS-QRCM, and MRG023/LCH/2013/ICMS). Publisher copyright: © 2015 American Chemical Society

PY - 2015/4/22

Y1 - 2015/4/22

N2 - A library of six luminescent Ir(III) complexes were synthesized and studied for their capacity to function as probes for G-quadruplex DNA. The novel Ir(III) complex 1 was discovered to be selective for G-quadruplex structures and was subsequently used for the construction of a label-free G-quadruplex-based ochratoxin A (OTA) sensing platform in aqueous solution. The assay exhibited linearity for OTA in the range of 0 to 60 nM (R2 = 0.9933), and the limit of detection for OTA was 5 nM. Furthermore, this assay was highly selective for OTA over its structurally related analogues.

AB - A library of six luminescent Ir(III) complexes were synthesized and studied for their capacity to function as probes for G-quadruplex DNA. The novel Ir(III) complex 1 was discovered to be selective for G-quadruplex structures and was subsequently used for the construction of a label-free G-quadruplex-based ochratoxin A (OTA) sensing platform in aqueous solution. The assay exhibited linearity for OTA in the range of 0 to 60 nM (R2 = 0.9933), and the limit of detection for OTA was 5 nM. Furthermore, this assay was highly selective for OTA over its structurally related analogues.

KW - G-quadruplex

KW - iridium(III) complex

KW - label-free

KW - luminescent

KW - ochratoxin A

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DO - 10.1021/acsami.5b01702

M3 - Journal article

C2 - 25836665

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SN - 1944-8244

VL - 7

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JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 15

ER -

Label-free luminescent switch-on probe for ochratoxin a detection using a G-quadruplex-selective iridium(III) complex

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