Label-free luminescent switch-on detection of endonuclease IV activity using a G-quadruplex-selective iridium(III) complex

Ka Ho Leung; Hong Zhang He; Wei Wang; Hai Jing Zhong; Daniel Shiu Hin Chan; Chung Hang Leung; Dik Lung Ma

doi:10.1021/am404314p

Label-free luminescent switch-on detection of endonuclease IV activity using a G-quadruplex-selective iridium(III) complex

Ka Ho Leung, Hong Zhang He, Wei Wang, Hai Jing Zhong, Daniel Shiu Hin Chan, Chung Hang Leung^*, Dik Lung Ma

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

56 Citations (Scopus)

Abstract

We report herein the synthesis and application of a novel G-quadruplex-selective luminescent iridium(III) complex [Ir(ppy) ₂(bcp)]⁺ (where ppy = 2-phenylpyridine and bcp = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) for the sensitive detection of apurinic/apyrimidinic (AP) endonuclease activity. Using endonuclease IV (Endo IV) as a model enzyme, a duplex DNA substrate containing a G-quadruplex-forming sequence is cleaved by Endo IV at the abasic site. This releases the G-quadruplex sequence, which folds into a G-quadruplex and is recognised by the G-quadruplex-selective iridium(III) complex with an enhanced luminescence response. The assay achieved high sensitivity and selectivity for Endo IV over other tested enzymes.

Original language	English
Pages (from-to)	12249-12253
Number of pages	5
Journal	ACS Applied Materials and Interfaces
Volume	5
Issue number	23
DOIs	https://doi.org/10.1021/am404314p
Publication status	Published - 11 Dec 2013

Scopus Subject Areas

General Materials Science

User-Defined Keywords

AP endonuclease
G-quadruplex
iridium(III) complex
label-free
luminescent

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10.1021/am404314p

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@article{cf71079a22684622b535a74ac34ba746,

title = "Label-free luminescent switch-on detection of endonuclease IV activity using a G-quadruplex-selective iridium(III) complex",

abstract = "We report herein the synthesis and application of a novel G-quadruplex-selective luminescent iridium(III) complex [Ir(ppy) 2(bcp)]+ (where ppy = 2-phenylpyridine and bcp = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) for the sensitive detection of apurinic/apyrimidinic (AP) endonuclease activity. Using endonuclease IV (Endo IV) as a model enzyme, a duplex DNA substrate containing a G-quadruplex-forming sequence is cleaved by Endo IV at the abasic site. This releases the G-quadruplex sequence, which folds into a G-quadruplex and is recognised by the G-quadruplex-selective iridium(III) complex with an enhanced luminescence response. The assay achieved high sensitivity and selectivity for Endo IV over other tested enzymes.",

keywords = "AP endonuclease, G-quadruplex, iridium(III) complex, label-free, luminescent",

author = "Leung, {Ka Ho} and He, {Hong Zhang} and Wei Wang and Zhong, {Hai Jing} and Chan, {Daniel Shiu Hin} and Leung, {Chung Hang} and Ma, {Dik Lung}",

note = "This work is supported by Hong Kong Baptist University (FRG2/12-13/021), the Centre for Cancer and Inflammation Research, the School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/ 11101212 and HMRF/13121482), the Research Grants Council (HKBU/201811, HKBU/204612, and HKBU/ 201913), the French National Research Agency/Research Grants Council Joint Research Scheme (103/2012/A3, 001/ 2012/A), the Science and Technology Development Fund, Macao SAR (001/2012/A and 103/2012/A3), and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH). ",

year = "2013",

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doi = "10.1021/am404314p",

language = "English",

volume = "5",

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journal = "ACS Applied Materials and Interfaces",

issn = "1944-8244",

publisher = "American Chemical Society",

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T1 - Label-free luminescent switch-on detection of endonuclease IV activity using a G-quadruplex-selective iridium(III) complex

AU - Leung, Ka Ho

AU - He, Hong Zhang

AU - Wang, Wei

AU - Zhong, Hai Jing

AU - Chan, Daniel Shiu Hin

AU - Leung, Chung Hang

AU - Ma, Dik Lung

N1 - This work is supported by Hong Kong Baptist University (FRG2/12-13/021), the Centre for Cancer and Inflammation Research, the School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/ 11101212 and HMRF/13121482), the Research Grants Council (HKBU/201811, HKBU/204612, and HKBU/ 201913), the French National Research Agency/Research Grants Council Joint Research Scheme (103/2012/A3, 001/ 2012/A), the Science and Technology Development Fund, Macao SAR (001/2012/A and 103/2012/A3), and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH).

PY - 2013/12/11

Y1 - 2013/12/11

N2 - We report herein the synthesis and application of a novel G-quadruplex-selective luminescent iridium(III) complex [Ir(ppy) 2(bcp)]+ (where ppy = 2-phenylpyridine and bcp = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) for the sensitive detection of apurinic/apyrimidinic (AP) endonuclease activity. Using endonuclease IV (Endo IV) as a model enzyme, a duplex DNA substrate containing a G-quadruplex-forming sequence is cleaved by Endo IV at the abasic site. This releases the G-quadruplex sequence, which folds into a G-quadruplex and is recognised by the G-quadruplex-selective iridium(III) complex with an enhanced luminescence response. The assay achieved high sensitivity and selectivity for Endo IV over other tested enzymes.

AB - We report herein the synthesis and application of a novel G-quadruplex-selective luminescent iridium(III) complex [Ir(ppy) 2(bcp)]+ (where ppy = 2-phenylpyridine and bcp = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) for the sensitive detection of apurinic/apyrimidinic (AP) endonuclease activity. Using endonuclease IV (Endo IV) as a model enzyme, a duplex DNA substrate containing a G-quadruplex-forming sequence is cleaved by Endo IV at the abasic site. This releases the G-quadruplex sequence, which folds into a G-quadruplex and is recognised by the G-quadruplex-selective iridium(III) complex with an enhanced luminescence response. The assay achieved high sensitivity and selectivity for Endo IV over other tested enzymes.

KW - AP endonuclease

KW - G-quadruplex

KW - iridium(III) complex

KW - label-free

KW - luminescent

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DO - 10.1021/am404314p

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AN - SCOPUS:84890536692

SN - 1944-8244

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SP - 12249

EP - 12253

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 23

ER -

Label-free luminescent switch-on detection of endonuclease IV activity using a G-quadruplex-selective iridium(III) complex

Abstract

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