Label-free luminescent switch-on detection of endonuclease IV activity using a G-quadruplex-selective iridium(III) complex

Ka Ho Leung, Hong Zhang He, Wei Wang, Hai Jing Zhong, Daniel Shiu Hin Chan, Chung Hang Leung*, Edmond Dik Lung MA

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

We report herein the synthesis and application of a novel G-quadruplex-selective luminescent iridium(III) complex [Ir(ppy) 2(bcp)]+ (where ppy = 2-phenylpyridine and bcp = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) for the sensitive detection of apurinic/apyrimidinic (AP) endonuclease activity. Using endonuclease IV (Endo IV) as a model enzyme, a duplex DNA substrate containing a G-quadruplex-forming sequence is cleaved by Endo IV at the abasic site. This releases the G-quadruplex sequence, which folds into a G-quadruplex and is recognised by the G-quadruplex-selective iridium(III) complex with an enhanced luminescence response. The assay achieved high sensitivity and selectivity for Endo IV over other tested enzymes.

Original languageEnglish
Pages (from-to)12249-12253
Number of pages5
JournalACS applied materials & interfaces
Volume5
Issue number23
DOIs
Publication statusPublished - 11 Dec 2013

Scopus Subject Areas

  • Materials Science(all)

User-Defined Keywords

  • AP endonuclease
  • G-quadruplex
  • iridium(III) complex
  • label-free
  • luminescent

Fingerprint

Dive into the research topics of 'Label-free luminescent switch-on detection of endonuclease IV activity using a G-quadruplex-selective iridium(III) complex'. Together they form a unique fingerprint.

Cite this