Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe

Modi Wang, Bingyong He, Lihua Lu, Chung Hang Leung, Jean Louis Mergny, Edmond Dik Lung MA*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We have synthesized a series of luminescent iridium(III) complexes and investigated their ability to act as luminescent split G-quadruplex probes. After screening, the iridium(III) complex 1 [Ir(2-phenylquinoline)2(3,4,7,8-tetramethyl-1,10-phenanthroline)]PF6 was validated as a highly-selective G-quadruplex probe and was utilized to construct a label-free intermolecular G-quadruplex-based assay for the selective and sensitive detection of LMP1 gene deletion. This "mix-and-detect" assay is simple and selective, and could detect down to 10nM of the target gene in aqueous solution with a linear range from 10 to 500nM. We also investigated the performance of our split G-quadruplex-based sensing platform for LMP1 gene deletion in the presence of cellular debris, demonstrating the robustness of this sensing system in biological samples. Comparative assays were also performed using either organic dyes or labeled oligonucleotides as signal-transducing agents.

Original languageEnglish
Pages (from-to)338-344
Number of pages7
JournalBiosensors and Bioelectronics
Volume70
DOIs
Publication statusPublished - 5 Aug 2015

Scopus Subject Areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

User-Defined Keywords

  • G-quadruplex
  • Gene deletion
  • Iridium(III) complex
  • Label-free detection
  • Luminescent probe

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