Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

Hong Zhang He; Ka Ho Leung; Wei Wang; Daniel Shiu Hin Chan; Chung Hang Leung; Dik Lung Ma

doi:10.1039/c3cc47444e

Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

Hong Zhang He
, Ka Ho Leung
, Wei Wang
, Daniel Shiu Hin Chan
, Chung Hang Leung
, Dik Lung Ma^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

120 Citations (Scopus)

Abstract

A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.

Original language	English
Pages (from-to)	5313-5315
Number of pages	3
Journal	Chemical Communications
Volume	50
Issue number	40
DOIs	https://doi.org/10.1039/c3cc47444e
Publication status	Published - 22 Apr 2014

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10.1039/c3cc47444e

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title = "Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe",

abstract = "A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.",

author = "He, \{Hong Zhang\} and Leung, \{Ka Ho\} and Wei Wang and Chan, \{Daniel Shiu Hin\} and Leung, \{Chung Hang\} and Ma, \{Dik Lung\}",

note = "This work is supported by Hong Kong Baptist University (FRG2/12-13/021), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482), the Research Grants Council (HKBU/201811, HKBU/204612, and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (103/2012/A3, 001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH). ",

year = "2014",

month = apr,

day = "22",

doi = "10.1039/c3cc47444e",

language = "English",

volume = "50",

pages = "5313--5315",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

AU - He, Hong Zhang

AU - Leung, Ka Ho

AU - Wang, Wei

AU - Chan, Daniel Shiu Hin

AU - Leung, Chung Hang

AU - Ma, Dik Lung

N1 - This work is supported by Hong Kong Baptist University (FRG2/12-13/021), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482), the Research Grants Council (HKBU/201811, HKBU/204612, and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (103/2012/A3, 001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH).

PY - 2014/4/22

Y1 - 2014/4/22

N2 - A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.

AB - A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.

UR - https://www.scopus.com/pages/publications/84899117570

U2 - 10.1039/c3cc47444e

DO - 10.1039/c3cc47444e

M3 - Journal article

C2 - 24336506

AN - SCOPUS:84899117570

SN - 1359-7345

VL - 50

SP - 5313

EP - 5315

JO - Chemical Communications

JF - Chemical Communications

IS - 40

ER -

Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

Abstract

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