Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

Hong Zhang He; Ka Ho Leung; Wei Wang; Daniel Shiu Hin Chan; Chung Hang Leung; Edmond Dik Lung MA

doi:10.1039/c3cc47444e

Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

Hong Zhang He, Ka Ho Leung, Wei Wang, Daniel Shiu Hin Chan, Chung Hang Leung, Edmond Dik Lung MA^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

119 Citations (Scopus)

Abstract

A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.

Original language	English
Pages (from-to)	5313-5315
Number of pages	3
Journal	Chemical Communications
Volume	50
Issue number	40
DOIs	https://doi.org/10.1039/c3cc47444e
Publication status	Published - 22 Apr 2014

Scopus Subject Areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.",

author = "He, {Hong Zhang} and Leung, {Ka Ho} and Wei Wang and Chan, {Daniel Shiu Hin} and Leung, {Chung Hang} and MA, {Edmond Dik Lung}",

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AU - He, Hong Zhang

AU - Leung, Ka Ho

AU - Wang, Wei

AU - Chan, Daniel Shiu Hin

AU - Leung, Chung Hang

AU - MA, Edmond Dik Lung

PY - 2014/4/22

Y1 - 2014/4/22

N2 - A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.

AB - A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.

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DO - 10.1039/c3cc47444e

M3 - Journal article

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AN - SCOPUS:84899117570

SN - 1359-7345

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JO - Chemical Communications

JF - Chemical Communications

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Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

Abstract

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