Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe

Ka Ho Leung; Hong Zhang He; Bingyong He; Hai Jing Zhong; Sheng Lin; Yi Tao Wang; Edmond Dik Lung Ma; Chung Hang Leung

doi:10.1039/c4sc03319a

Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe

Ka Ho Leung
, Hong Zhang He
, Bingyong He
, Hai Jing Zhong
, Sheng Lin
, Yi Tao Wang
, Edmond Dik Lung Ma^*
, Chung Hang Leung^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

138 Citations (Scopus)

Abstract

A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex 9, [Ir(phq)₂(phen)]PF₆ (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.

Original language	English
Pages (from-to)	2166-2171
Number of pages	6
Journal	Chemical Science
Volume	6
Issue number	4
Early online date	25 Nov 2014
DOIs	https://doi.org/10.1039/c4sc03319a
Publication status	Published - 1 Apr 2015

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title = "Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe",

abstract = "A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex 9, [Ir(phq)2(phen)]PF6 (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity. ",

author = "Leung, \{Ka Ho\} and He, \{Hong Zhang\} and Bingyong He and Zhong, \{Hai Jing\} and Sheng Lin and Wang, \{Yi Tao\} and Ma, \{Edmond Dik Lung\} and Leung, \{Chung Hang\}",

note = "This work is supported by Hong Kong Baptist University (FRG2/13-14/008), the Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482), the Research Grants Council (HKBU/201811, HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the State Key Laboratory of Synthetic Chemistry, the Science and Technology Development Fund, Macao SAR (103/2012/A3) and the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH, MYRG121(Y3-L2)-ICMS12-LCH and MRG023/LCH/2013/ICMS).",

year = "2015",

month = apr,

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doi = "10.1039/c4sc03319a",

language = "English",

volume = "6",

pages = "2166--2171",

journal = "Chemical Science",

issn = "2041-6520",

publisher = "Royal Society of Chemistry",

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T1 - Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe

AU - Leung, Ka Ho

AU - He, Hong Zhang

AU - He, Bingyong

AU - Zhong, Hai Jing

AU - Lin, Sheng

AU - Wang, Yi Tao

AU - Ma, Edmond Dik Lung

AU - Leung, Chung Hang

N1 - This work is supported by Hong Kong Baptist University (FRG2/13-14/008), the Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482), the Research Grants Council (HKBU/201811, HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the State Key Laboratory of Synthetic Chemistry, the Science and Technology Development Fund, Macao SAR (103/2012/A3) and the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH, MYRG121(Y3-L2)-ICMS12-LCH and MRG023/LCH/2013/ICMS).

PY - 2015/4/1

Y1 - 2015/4/1

N2 - A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex 9, [Ir(phq)2(phen)]PF6 (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.

AB - A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex 9, [Ir(phq)2(phen)]PF6 (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.

UR - https://www.scopus.com/pages/publications/84924943642

U2 - 10.1039/c4sc03319a

DO - 10.1039/c4sc03319a

M3 - Journal article

AN - SCOPUS:84924943642

SN - 2041-6520

VL - 6

SP - 2166

EP - 2171

JO - Chemical Science

JF - Chemical Science

IS - 4

ER -

Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe

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