Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

Zhenyu Lin; Lifen Chen; Guiyun Zhang; Qida Liu; Bin Qiu; Zongwei CAI; Guonan Chen

doi:10.1039/c1an15856b

Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

Zhenyu Lin^*
, Lifen Chen
, Guiyun Zhang
, Qida Liu
, Bin Qiu
, Zongwei CAI
, Guonan Chen

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

95 Citations (Scopus)

Abstract

A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10^-8 to 1.0 × 10^-11 mol L^-1, with a detection limit of 2.0 × 10^-12 mol L^-1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.

Original language	English
Pages (from-to)	819-822
Number of pages	4
Journal	Analyst
Volume	137
Issue number	4
DOIs	https://doi.org/10.1039/c1an15856b
Publication status	Published - 21 Feb 2012

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10.1039/c1an15856b

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@article{d451fd8d83804f4fbd0c04c7daa3dc09,

title = "Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol",

abstract = "A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10-8 to 1.0 × 10-11 mol L-1, with a detection limit of 2.0 × 10-12 mol L-1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.",

author = "Zhenyu Lin and Lifen Chen and Guiyun Zhang and Qida Liu and Bin Qiu and Zongwei CAI and Guonan Chen",

note = "This project was financially supported by the National Basic Research Program of China (No. 2010CB732403), National Nature Sciences Funding of China (20735002, 40940026, 20905013), and Supported by Program for New Century Excellent Talents in Fujian Province University (JA10011).",

year = "2012",

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doi = "10.1039/c1an15856b",

language = "English",

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T1 - Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

AU - Lin, Zhenyu

AU - Chen, Lifen

AU - Zhang, Guiyun

AU - Liu, Qida

AU - Qiu, Bin

AU - CAI, Zongwei

AU - Chen, Guonan

N1 - This project was financially supported by the National Basic Research Program of China (No. 2010CB732403), National Nature Sciences Funding of China (20735002, 40940026, 20905013), and Supported by Program for New Century Excellent Talents in Fujian Province University (JA10011).

PY - 2012/2/21

Y1 - 2012/2/21

N2 - A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10-8 to 1.0 × 10-11 mol L-1, with a detection limit of 2.0 × 10-12 mol L-1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.

AB - A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10-8 to 1.0 × 10-11 mol L-1, with a detection limit of 2.0 × 10-12 mol L-1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.

UR - https://www.scopus.com/pages/publications/84862959186

U2 - 10.1039/c1an15856b

DO - 10.1039/c1an15856b

M3 - Journal article

AN - SCOPUS:84862959186

SN - 0003-2654

VL - 137

SP - 819

EP - 822

JO - Analyst

JF - Analyst

IS - 4

ER -

Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

Abstract

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