Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

Zhenyu Lin*, Lifen Chen, Guiyun Zhang, Qida Liu, Bin Qiu, Zongwei CAI, Guonan Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

77 Citations (Scopus)

Abstract

A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10-8 to 1.0 × 10-11 mol L-1, with a detection limit of 2.0 × 10-12 mol L-1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.

Original languageEnglish
Pages (from-to)819-822
Number of pages4
JournalThe Analyst
Volume137
Issue number4
DOIs
Publication statusPublished - 21 Feb 2012

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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