Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

Zhenyu Lin; Lifen Chen; Guiyun Zhang; Qida Liu; Bin Qiu; Zongwei CAI; Guonan Chen

doi:10.1039/c1an15856b

Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

Zhenyu Lin^*, Lifen Chen, Guiyun Zhang, Qida Liu, Bin Qiu, Zongwei CAI, Guonan Chen

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

93 Citations (Scopus)

Abstract

A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10^-8 to 1.0 × 10^-11 mol L^-1, with a detection limit of 2.0 × 10^-12 mol L^-1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.

Original language	English
Pages (from-to)	819-822
Number of pages	4
Journal	Analyst
Volume	137
Issue number	4
DOIs	https://doi.org/10.1039/c1an15856b
Publication status	Published - 21 Feb 2012

Scopus Subject Areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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title = "Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol",

abstract = "A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10-8 to 1.0 × 10-11 mol L-1, with a detection limit of 2.0 × 10-12 mol L-1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.",

author = "Zhenyu Lin and Lifen Chen and Guiyun Zhang and Qida Liu and Bin Qiu and Zongwei CAI and Guonan Chen",

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T1 - Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

AU - Lin, Zhenyu

AU - Chen, Lifen

AU - Zhang, Guiyun

AU - Liu, Qida

AU - Qiu, Bin

AU - CAI, Zongwei

AU - Chen, Guonan

PY - 2012/2/21

Y1 - 2012/2/21

N2 - A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10-8 to 1.0 × 10-11 mol L-1, with a detection limit of 2.0 × 10-12 mol L-1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.

AB - A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10-8 to 1.0 × 10-11 mol L-1, with a detection limit of 2.0 × 10-12 mol L-1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.

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Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

Abstract

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