Kinetic modeling of hydrogen and L-lactic acid production by Thermotoga neapolitana via capnophilic lactic fermentation of starch

Nirakar PRADHAN*, Giuliana d'Ippolito, Laura Dipasquale, Giovanni Esposito, Antonio Panico, Piet N.L. Lens, Angelo Fontana

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This study investigated the feasibility of hydrogen (H2) and L-lactic acid production from starch under capnophilic lactic fermentation (CLF) conditions by using Thermotoga neapolitana. Batch experiments were performed in 120 mL serum bottles and a 3 L pH-controlled continuous stirred-tank reactors (CSTR) system with potato and wheat starch as the substrates. A H2 yield of 3.34 (±0.17) and 2.79 (±0.17) mol H2/mol of glucose eq. was achieved with, respectively, potato and wheat starch. In the presence of CO2, L-lactic acid production by the acetyl-CoA carboxylation was significantly higher for the potato starch (0.88 ± 0.39 mol lactic acid/mol glucose eq.) than wheat starch (0.33 ± 0.11 mol lactic acid/mol glucose eq.). A kinetic model was applied to simulate and predict the T. neapolitana metabolic profile and bioreactor performance under CLF conditions. The CLF-based starch fermentation suggests a new direction to biotransform agri-food waste into biofuels and valuable biochemicals.

Original languageEnglish
Article number125127
JournalBioresource Technology
Volume332
DOIs
Publication statusPublished - Jul 2021

Scopus Subject Areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

User-Defined Keywords

  • Agri-food waste
  • Fermentation
  • Hydrogen
  • Lactic acid
  • Thermotoga neapolitana

Fingerprint

Dive into the research topics of 'Kinetic modeling of hydrogen and L-lactic acid production by Thermotoga neapolitana via capnophilic lactic fermentation of starch'. Together they form a unique fingerprint.

Cite this