Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense

Xiao Qiang Han; Ben Chung Lap Chan; Cai Xia Dong; Yin Hua Yang; Chun Hay Ko; Grace Gar-Lee Yue; Dan Chen; Chun Kwok Wong; Clara Bik-San Lau; Peng Fei Tu; Pang Chui Shaw; Kwok Pui Fung; Ping Chung Leung; Wendy W L HSIAO; Simon Q B HAN

doi:10.1021/jf205056u

Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense

Xiao Qiang Han, Ben Chung Lap Chan, Cai Xia Dong, Yin Hua Yang, Chun Hay Ko, Grace Gar-Lee Yue, Dan Chen, Chun Kwok Wong, Clara Bik-San Lau, Peng Fei Tu^*, Pang Chui Shaw, Kwok Pui Fung, Ping Chung Leung, Wendy W L HSIAO, Simon Q B HAN

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

68 Citations (Scopus)

Abstract

A polysaccharide (GSP-6B) with a molecular mass of 1.86 ×10 ⁶ Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-d- glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β- d-glucopyranosyl residues, (1→3)-linked-β-d-glucopyranosyl residues, and nonreducing end β-d-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1β and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.

Original language	English
Pages (from-to)	4276-4281
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	60
Issue number	17
DOIs	https://doi.org/10.1021/jf205056u
Publication status	Published - 2 May 2012

Scopus Subject Areas

Chemistry(all)
Agricultural and Biological Sciences(all)

User-Defined Keywords

cytokine
dendritic cell
Ganoderma sinense
immunological activity
polysaccharide

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10.1021/jf205056u

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Han, X. Q., Chan, B. C. L., Dong, C. X., Yang, Y. H., Ko, C. H., Gar-Lee Yue, G., Chen, D., Wong, C. K., Bik-San Lau, C., Tu, P. F., Shaw, P. C., Fung, K. P., Leung, P. C., HSIAO, W. W. L., & HAN, S. Q. B. (2012). Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense. Journal of Agricultural and Food Chemistry, 60(17), 4276-4281. https://doi.org/10.1021/jf205056u

@article{2f7bcd4940f24ea691ef3ce5b7bf33ce,

title = "Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense",

abstract = "A polysaccharide (GSP-6B) with a molecular mass of 1.86 ×10 6 Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-d- glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β- d-glucopyranosyl residues, (1→3)-linked-β-d-glucopyranosyl residues, and nonreducing end β-d-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1β and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.",

keywords = "cytokine, dendritic cell, Ganoderma sinense, immunological activity, polysaccharide",

author = "Han, {Xiao Qiang} and Chan, {Ben Chung Lap} and Dong, {Cai Xia} and Yang, {Yin Hua} and Ko, {Chun Hay} and {Gar-Lee Yue}, Grace and Dan Chen and Wong, {Chun Kwok} and {Bik-San Lau}, Clara and Tu, {Peng Fei} and Shaw, {Pang Chui} and Fung, {Kwok Pui} and Leung, {Ping Chung} and HSIAO, {Wendy W L} and HAN, {Simon Q B}",

note = "This research is funded by the Innovation and Technology Fund (ITS/311/09 and InP/108/10) of the Government of the Hong Kong Special Administrative Region and Hong Kong Baptist University (FRG2/11-12/048). ",

year = "2012",

month = may,

day = "2",

doi = "10.1021/jf205056u",

language = "English",

volume = "60",

pages = "4276--4281",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "17",

}

Han, XQ, Chan, BCL, Dong, CX, Yang, YH, Ko, CH, Gar-Lee Yue, G, Chen, D, Wong, CK, Bik-San Lau, C, Tu, PF, Shaw, PC, Fung, KP, Leung, PC, HSIAO, WWL & HAN, SQB 2012, 'Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense', Journal of Agricultural and Food Chemistry, vol. 60, no. 17, pp. 4276-4281. https://doi.org/10.1021/jf205056u

Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense. / Han, Xiao Qiang; Chan, Ben Chung Lap; Dong, Cai Xia et al.
In: Journal of Agricultural and Food Chemistry, Vol. 60, No. 17, 02.05.2012, p. 4276-4281.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense

AU - Han, Xiao Qiang

AU - Chan, Ben Chung Lap

AU - Dong, Cai Xia

AU - Yang, Yin Hua

AU - Ko, Chun Hay

AU - Gar-Lee Yue, Grace

AU - Chen, Dan

AU - Wong, Chun Kwok

AU - Bik-San Lau, Clara

AU - Tu, Peng Fei

AU - Shaw, Pang Chui

AU - Fung, Kwok Pui

AU - Leung, Ping Chung

AU - HSIAO, Wendy W L

AU - HAN, Simon Q B

N1 - This research is funded by the Innovation and Technology Fund (ITS/311/09 and InP/108/10) of the Government of the Hong Kong Special Administrative Region and Hong Kong Baptist University (FRG2/11-12/048).

PY - 2012/5/2

Y1 - 2012/5/2

N2 - A polysaccharide (GSP-6B) with a molecular mass of 1.86 ×10 6 Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-d- glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β- d-glucopyranosyl residues, (1→3)-linked-β-d-glucopyranosyl residues, and nonreducing end β-d-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1β and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.

AB - A polysaccharide (GSP-6B) with a molecular mass of 1.86 ×10 6 Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-d- glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β- d-glucopyranosyl residues, (1→3)-linked-β-d-glucopyranosyl residues, and nonreducing end β-d-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1β and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.

KW - cytokine

KW - dendritic cell

KW - Ganoderma sinense

KW - immunological activity

KW - polysaccharide

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U2 - 10.1021/jf205056u

DO - 10.1021/jf205056u

M3 - Journal article

C2 - 22500548

AN - SCOPUS:84860442496

SN - 0021-8561

VL - 60

SP - 4276

EP - 4281

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 17

ER -

Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense

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