Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense

Xiao Qiang Han, Ben Chung Lap Chan, Cai Xia Dong, Yin Hua Yang, Chun Hay Ko, Grace Gar-Lee Yue, Dan Chen, Chun Kwok Wong, Clara Bik-San Lau, Peng Fei Tu*, Pang Chui Shaw, Kwok Pui Fung, Ping Chung Leung, Wendy W L HSIAO, Simon Q B HAN

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

A polysaccharide (GSP-6B) with a molecular mass of 1.86 ×10 6 Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-d- glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β- d-glucopyranosyl residues, (1→3)-linked-β-d-glucopyranosyl residues, and nonreducing end β-d-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1β and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.

Original languageEnglish
Pages (from-to)4276-4281
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number17
DOIs
Publication statusPublished - 2 May 2012

Scopus Subject Areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

User-Defined Keywords

  • cytokine
  • dendritic cell
  • Ganoderma sinense
  • immunological activity
  • polysaccharide

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