Isolation of AtNUDT5 gene promoter and characterization of its activity in transgenic Arabidopsis thaliana

Xiu Chun Zhang, Mei Ying Li, Meng Bin Ruan, Yiji XIA, Kun Xin Wu*, Ming Peng

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

3 Citations (Scopus)

Abstract

AtNUDT5 is a cytosol Nudix that catalyzes the hydrolysis of a variety of substrates. In this report, a 1,387-bp 5'-flanking region of the AtNUDT5 gene was isolated from Arabidopsis thaliana. The tissue-specific activity of the 5'-flanking region was investigated by using the GUS gene as a reporter in transgenic A. thaliana plants. Weak GUS activity appeared in vascular tissues of young plants, strong GUS activity appeared in the axial roots, but no GUS activity was observed in the root cap, lateral roots, rosette leaf, mature silique and reproductive tissues such as stamen, pistil, and petal. Furthermore, by using these transgenic A. thaliana plants, results of the histochemical staining and fluorometric assays of GUS activity showed that the AtNUDT5 promoter can be activated by both avirulent Pst avrRpm1 and virulent Pst strains at 5 h post-infiltration and that the activity of AtNUDT5 promoter increased significantly at 24 h post-infiltration. Taken together, our results demonstrated that the AtNUDT5 promoter is pathogen-responsive. The promoter may be used to develop transgenic plants with an increased tolerance to pathogenic stresses.

Original languageEnglish
Pages (from-to)1557-1565
Number of pages9
JournalApplied Biochemistry and Biotechnology
Volume169
Issue number5
DOIs
Publication statusPublished - Mar 2013

Scopus Subject Areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

User-Defined Keywords

  • AtNUDT5 promoter
  • Pathogen-responsive
  • Pseudomonas syringae pv. Tomato DC3000
  • Transgenic Arabidopsis thaliana

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