TY - JOUR
T1 - Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation
AU - Ma, Fengwei
AU - Liu, Hongbing
AU - Xu, Su
AU - Cheng, Yongyou
AU - Fei, Qiang
AU - Chen, Haijiang
N1 - Funding Information:
This research was supported by Department of Science and Technology of Guizhou Province ([2019]1013), the National Natural Science Foundation of China (No. 21672206), General Research Fund (12100615, 22100014), Health and Medical Research Fund (11122531, 14150521), and Hong Kong Baptist University (RC-start up grant, MPCF-002-2016/2017, and FRG2/16-17/002).
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/2/28
Y1 - 2021/2/28
N2 - A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith. LCP-05 was an acidic polysaccharide with a molecular weight of approximately 8.9 kDa. Monosaccharide composition analysis indicated that LCP-05 was composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara in a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22. The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues. The side chains are terminated primarily with the Araf and Glcp residues. LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
AB - A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith. LCP-05 was an acidic polysaccharide with a molecular weight of approximately 8.9 kDa. Monosaccharide composition analysis indicated that LCP-05 was composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara in a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22. The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues. The side chains are terminated primarily with the Araf and Glcp residues. LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
KW - Acidic polysaccharide
KW - Immunomodulatory activity
KW - Leucosceptrum canum Smith
KW - Purification
KW - Structure
UR - http://www.scopus.com/inward/record.url?scp=85098855139&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2021.01.009
DO - 10.1016/j.ijbiomac.2021.01.009
M3 - Journal article
C2 - 33421465
AN - SCOPUS:85098855139
SN - 0141-8130
VL - 171
SP - 177
EP - 184
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -
