Isolation of a methylobacterium organophilium strain, and its application to a methanol biosensor

Chungui Zhao; Haipeng Li; Jun Zheng; Liang Chen; Feng Li; Suping Yang; Chuan Dong; Martin M.F. Choi

doi:10.1007/s00604-009-0214-7

Isolation of a methylobacterium organophilium strain, and its application to a methanol biosensor

Chungui Zhao, Haipeng Li, Jun Zheng, Liang Chen, Feng Li, Suping Yang, Chuan Dong^*, Martin M.F. Choi

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

4 Citations (Scopus)

Abstract

A methanol-utilizing strain (ME25) using methanol (MeOH) as the sole carbon source has been isolated from methane-generating pits. ME25 was identified as Methylobacterium organophilium by its physiological characteristics and 16SrDNA sequence. A MeOH biosensor was then developed by immobilizing ME25 along with sensor for dissolved oxygen (O₂). Its response is based on the depletion of O₂ following oxidation of MeOH by the bacteria. The decrease in O₂ is linearly related to the MeOH concentration in the range from 1.6 mg·L^-1 to 4800 mg·L^-1 and the detection limit for MeOH is 0.27 mg·L^-1. The response time of the biosensor is around 20 min.

Original language	English
Pages (from-to)	67-73
Number of pages	7
Journal	Microchimica Acta
Volume	167
Issue number	1
DOIs	https://doi.org/10.1007/s00604-009-0214-7
Publication status	Published - Oct 2009

Scopus Subject Areas

Analytical Chemistry

User-Defined Keywords

Dissolved oxygen
Methane-generating pits
Methanol biosensing system
Methanol determination
Methylobacterium organophilium

Access to Document

10.1007/s00604-009-0214-7

Cite this

@article{098a3bd908f64aab8eb5265a5f9b5e55,

title = "Isolation of a methylobacterium organophilium strain, and its application to a methanol biosensor",

abstract = "A methanol-utilizing strain (ME25) using methanol (MeOH) as the sole carbon source has been isolated from methane-generating pits. ME25 was identified as Methylobacterium organophilium by its physiological characteristics and 16SrDNA sequence. A MeOH biosensor was then developed by immobilizing ME25 along with sensor for dissolved oxygen (O2). Its response is based on the depletion of O2 following oxidation of MeOH by the bacteria. The decrease in O2 is linearly related to the MeOH concentration in the range from 1.6 mg·L-1 to 4800 mg·L-1 and the detection limit for MeOH is 0.27 mg·L-1. The response time of the biosensor is around 20 min.",

keywords = "Dissolved oxygen, Methane-generating pits, Methanol biosensing system, Methanol determination, Methylobacterium organophilium",

author = "Chungui Zhao and Haipeng Li and Jun Zheng and Liang Chen and Feng Li and Suping Yang and Chuan Dong and Choi, {Martin M.F.}",

year = "2009",

month = oct,

doi = "10.1007/s00604-009-0214-7",

language = "English",

volume = "167",

pages = "67--73",

journal = "Microchimica Acta",

issn = "0026-3672",

publisher = "Springer-Verlag Wien",

number = "1",

}

TY - JOUR

T1 - Isolation of a methylobacterium organophilium strain, and its application to a methanol biosensor

AU - Zhao, Chungui

AU - Li, Haipeng

AU - Zheng, Jun

AU - Chen, Liang

AU - Li, Feng

AU - Yang, Suping

AU - Dong, Chuan

AU - Choi, Martin M.F.

PY - 2009/10

Y1 - 2009/10

N2 - A methanol-utilizing strain (ME25) using methanol (MeOH) as the sole carbon source has been isolated from methane-generating pits. ME25 was identified as Methylobacterium organophilium by its physiological characteristics and 16SrDNA sequence. A MeOH biosensor was then developed by immobilizing ME25 along with sensor for dissolved oxygen (O2). Its response is based on the depletion of O2 following oxidation of MeOH by the bacteria. The decrease in O2 is linearly related to the MeOH concentration in the range from 1.6 mg·L-1 to 4800 mg·L-1 and the detection limit for MeOH is 0.27 mg·L-1. The response time of the biosensor is around 20 min.

AB - A methanol-utilizing strain (ME25) using methanol (MeOH) as the sole carbon source has been isolated from methane-generating pits. ME25 was identified as Methylobacterium organophilium by its physiological characteristics and 16SrDNA sequence. A MeOH biosensor was then developed by immobilizing ME25 along with sensor for dissolved oxygen (O2). Its response is based on the depletion of O2 following oxidation of MeOH by the bacteria. The decrease in O2 is linearly related to the MeOH concentration in the range from 1.6 mg·L-1 to 4800 mg·L-1 and the detection limit for MeOH is 0.27 mg·L-1. The response time of the biosensor is around 20 min.

KW - Dissolved oxygen

KW - Methane-generating pits

KW - Methanol biosensing system

KW - Methanol determination

KW - Methylobacterium organophilium

UR - http://www.scopus.com/inward/record.url?scp=70350719215&partnerID=8YFLogxK

U2 - 10.1007/s00604-009-0214-7

DO - 10.1007/s00604-009-0214-7

M3 - Journal article

AN - SCOPUS:70350719215

SN - 0026-3672

VL - 167

SP - 67

EP - 73

JO - Microchimica Acta

JF - Microchimica Acta

IS - 1

ER -

Isolation of a methylobacterium organophilium strain, and its application to a methanol biosensor

Abstract

Scopus Subject Areas

User-Defined Keywords

Access to Document

Other files and links

Fingerprint

Cite this