Isolation of a methylobacterium organophilium strain, and its application to a methanol biosensor

Chungui Zhao, Haipeng Li, Jun Zheng, Liang Chen, Feng Li, Suping Yang, Chuan Dong*, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

A methanol-utilizing strain (ME25) using methanol (MeOH) as the sole carbon source has been isolated from methane-generating pits. ME25 was identified as Methylobacterium organophilium by its physiological characteristics and 16SrDNA sequence. A MeOH biosensor was then developed by immobilizing ME25 along with sensor for dissolved oxygen (O2). Its response is based on the depletion of O2 following oxidation of MeOH by the bacteria. The decrease in O2 is linearly related to the MeOH concentration in the range from 1.6 mg·L-1 to 4800 mg·L-1 and the detection limit for MeOH is 0.27 mg·L-1. The response time of the biosensor is around 20 min.

Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalMikrochimica Acta
Volume167
Issue number1
DOIs
Publication statusPublished - Oct 2009

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Dissolved oxygen
  • Methane-generating pits
  • Methanol biosensing system
  • Methanol determination
  • Methylobacterium organophilium

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