TY - JOUR
T1 - Isolation and structural characterization of an immunostimulating polysaccharide from fuzi, Aconitum carmichaeli
AU - Zhao, Chi
AU - LI, Min
AU - Luo, Yifan
AU - Wu, Weikang
N1 - Funding Information:
This work was supported by the National Nature Science Foundation of China (No. 30070930).
PY - 2006/3/20
Y1 - 2006/3/20
N2 - A water-soluble polysaccharide named as FPS-1 was isolated from fuzi, the root of Aconitum carmichaeli Debx. by hot-water extraction, anion-exchange and gel-permeation chromatography and tested for its pharmacological activities. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, methylation analysis and GLC-MS. Based on the data obtained, FPS-1 was determined to be an α-(1→6)-d-glucan, with a weight-average molecular weight of about 14,000 Da. The glucan is highly branched with a single glucose at the C-3 position every four residues, on average, along the main chain. In immunopharmacological studies, FPS-1 showed potent stimulating effects on murine lymphocyte proliferation induced by concanavalin A or lipopolysaccharide both in vitro and in vivo as well as on splenocyte antibody production.
AB - A water-soluble polysaccharide named as FPS-1 was isolated from fuzi, the root of Aconitum carmichaeli Debx. by hot-water extraction, anion-exchange and gel-permeation chromatography and tested for its pharmacological activities. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, methylation analysis and GLC-MS. Based on the data obtained, FPS-1 was determined to be an α-(1→6)-d-glucan, with a weight-average molecular weight of about 14,000 Da. The glucan is highly branched with a single glucose at the C-3 position every four residues, on average, along the main chain. In immunopharmacological studies, FPS-1 showed potent stimulating effects on murine lymphocyte proliferation induced by concanavalin A or lipopolysaccharide both in vitro and in vivo as well as on splenocyte antibody production.
KW - α-D-Glucan
KW - Aconitum carmichaeli
KW - Fuzi
KW - Lymphocyte proliferation
KW - Ranunculaceae
KW - Structure
UR - http://www.scopus.com/inward/record.url?scp=32044435384&partnerID=8YFLogxK
U2 - 10.1016/j.carres.2005.11.032
DO - 10.1016/j.carres.2005.11.032
M3 - Journal article
C2 - 16406277
AN - SCOPUS:32044435384
SN - 0008-6215
VL - 341
SP - 485
EP - 491
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 4
ER -