Isolation and characterization of crude-oil-degrading bacteria from oil-water mixture in Dagang oilfield, China

Haijun Liu, Jun Yao*, Zhimin Yuan, Yifan Shang, Huilun Chen, Fei Wang, Kanaji Masakorala, Chan Yu, Minmin Cai, Ruth E. Blake, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Isolating novel crude-oil-degrading bacteria from oil-water mixture of oil production well and evaluating their degradation capacities are vitally important in the remediation of oil-polluted environments and crude oil exploitation. According to the molecular screening with degenerate primers of alkane hydroxylase gene (alk B), a strain Acinetobacter sp. LS-1 with alk B gene was isolated. This strain exhibited a 99.9% similarity with genus Acinetobacter. This alk B gene which is one of the key enzymes of metabolic process was identified. This gene sequence showed 98% similarity of its nucleotide and related amino acids to the genus Marinobacter but exhibited below 70% similarity to the genus Acinetobacter. This phylogenetic analysis indicated that alk B may have been transferred horizontally between bacteria. The isolated strain could utilize crude oil as the sole carbon, achieving a high degradation (70.3%) in 7 days. Microcalorimetric analysis of the metabolic processes for hexadecane degradation also demonstrated the ability of this strain to utilize hydrocarbons. Thus, this strain enables to degrade hydrocarbons as the sole carbon source from the gene level, combined with material and energy metabolism. These findings will benefit this strain in the remediation of oil-polluted environments and oil exploitation.

Original languageEnglish
Pages (from-to)52-59
Number of pages8
JournalInternational Biodeterioration and Biodegradation
Volume87
DOIs
Publication statusPublished - Feb 2014

Scopus Subject Areas

  • Microbiology
  • Biomaterials
  • Waste Management and Disposal

User-Defined Keywords

  • Acinetobacter
  • Alkane hydroxylase gene
  • Crude oil
  • Degradation
  • Thermal effect

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