Isolation and characterization of a novel ribosome-inactivating protein, β-kirilowin, from the seeds of Trichosanthes kirilowii

T. X. Dong, T. B. Ng, H. W. Yeung, Ricky N S WONG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

A novel ribosome-inactivating protein designated β-kirilowin was isolated from the seeds of Trichosanthes kirilowii. The proteins exhibited strong abortifacient activity in pregnant mice and produced a single band after SDS-polyacrylamide gel electrophoresis with an apparent mol. wt. of 27,500 daltons. It was active in inhibiting the cell-free translation system with an ID50 of about 1.8 ng/ml. No cross-reactivity with trichosanthin could be detected by immunodiffusion. Sequence comparison of the first 10 residues of β-kirilowin with ribosome-inactivating proteins isolated from T. kirilowii such as trichosanthin, trichokirin and karasurin indicated 70%, 60% and 70% identity, respectively.

Original languageEnglish
Pages (from-to)387-393
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume199
Issue number1
DOIs
Publication statusPublished - 28 Feb 1994

Scopus Subject Areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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