Isolation and Anticancer Progression Evaluation of the Chemical Constituents from Bridelia balansae Tutcher

Lihan Zhao, Wen-Jian Xie, Yin-Xiao Du, Yi-Xuan Xia, Kang-Lun Liu, Chuen Fai Ku, Zihao Ou, Ming-Zhong Wang*, Hong-Jie Zhang*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review


The dichloromethane extract of the roots of Bridelia balansae Tutcher (Phyllanthaceae) was found to show potential anticancer activity against HCT116 colorectal cancer cell. Our bioassay-guided phytochemical investigation of the roots of B. balansae led to the identification of 14 compounds including seven lignans (1–7), three phenylbenzene derivatives (8–10), two flavanone (11–12), and two triterpenoids (13–14). Among them, 4′-demethyl-4-deoxypodophyllotoxin (1) is the first aryltetralin lignan compound identified from this plant species. In addition, the stereochemistry of 1 was validated by X-ray crystallography for the first time, and its distinguished cytotoxic effect on HCT116 cells with an IC50 value at 20 nM was induced via an apoptosis induction mechanism. Compound 1 could also significantly decrease the migration rate of HCT116 cells, indicating its potential application against cancer metastasis. The western blot analysis showed that 1 has the potential to inhibit cell proliferation and metastasis. Treatment of 1 resulted in the downregulation of matrix metalloproteinases 2 (MMP2) and p-Akt, while p21 was upregulated. Collectively, the present study on the phytochemical and biological profile of B. balansae has determined the plant as a useful source to produce promising anticancer lead compounds.

Original languageEnglish
Article number6165
Number of pages13
Issue number16
Publication statusPublished - 2 Aug 2023

Scopus Subject Areas

  • Pharmaceutical Science
  • Organic Chemistry
  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Drug Discovery
  • Physical and Theoretical Chemistry

User-Defined Keywords

  • Bridelia balansae
  • lignan compounds
  • colorectal cancer
  • cytotoxicity
  • apoptosis


Dive into the research topics of 'Isolation and Anticancer Progression Evaluation of the Chemical Constituents from Bridelia balansae Tutcher'. Together they form a unique fingerprint.

Cite this