Is phospholipid-saturated alkyl column a convenient replacement for immobilized-artificial-membrane?

Hai Bin Luo; Chuanqi Zheng; Yuen Kit Cheng

doi:10.1016/j.chroma.2007.10.093

Is phospholipid-saturated alkyl column a convenient replacement for immobilized-artificial-membrane?

Hai Bin Luo, Chuanqi Zheng, Yuen Kit Cheng^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

10 Citations (Scopus)

Abstract

Three phosphatidylcholine (PC)-saturated C₈/C₁₈ stationary phases prepared using biologically representative membrane lipids (purchasable L-α-PC and pure 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine) have been developed to compare with IAM (immobilized-artificial-membrane) and C₈/C₁₈ columns. These PC-coated stationary phases were found to be stable and reproducible for retention experiments. The retention characteristics of nucleobases on these coated phases deviate significantly from those on the IAM counterpart, but surprisingly similar to those of the underlying C₈/C₁₈ columns. An inter-phase model has been proposed and explored for interpretation of the results.

Original language	English
Pages (from-to)	100-106
Number of pages	7
Journal	Journal of Chromatography A
Volume	1176
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2007.10.093
Publication status	Published - 28 Dec 2007

Scopus Subject Areas

Analytical Chemistry
Biochemistry
Organic Chemistry

User-Defined Keywords

Dynamic coating
IAM
Nucleobases
Phosphatidylcholine/PC
Retention

Access to Document

10.1016/j.chroma.2007.10.093

Cite this

@article{ac3d9cc8aa7c4f41816e5a5174a53390,

title = "Is phospholipid-saturated alkyl column a convenient replacement for immobilized-artificial-membrane?",

abstract = "Three phosphatidylcholine (PC)-saturated C8/C18 stationary phases prepared using biologically representative membrane lipids (purchasable L-α-PC and pure 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine) have been developed to compare with IAM (immobilized-artificial-membrane) and C8/C18 columns. These PC-coated stationary phases were found to be stable and reproducible for retention experiments. The retention characteristics of nucleobases on these coated phases deviate significantly from those on the IAM counterpart, but surprisingly similar to those of the underlying C8/C18 columns. An inter-phase model has been proposed and explored for interpretation of the results.",

keywords = "Dynamic coating, IAM, Nucleobases, Phosphatidylcholine/PC, Retention",

author = "Luo, {Hai Bin} and Chuanqi Zheng and Cheng, {Yuen Kit}",

note = "Funding Information: We cordially acknowledge the Hong Kong RGC CERG Grant (HKBU 2035/02P) making this work possible. ",

year = "2007",

month = dec,

day = "28",

doi = "10.1016/j.chroma.2007.10.093",

language = "English",

volume = "1176",

pages = "100--106",

journal = "Journal of Chromatography A",

issn = "0021-9673",