Iridium(III) complexes with 1,10-phenanthroline-based N^N ligands as highly selective luminescent G-quadruplex probes and application for switch-on ribonuclease H detection

Lihua Lu; Wanhe Wang; Chao Yang; Tian Shu Kang; Chung Hang Leung; Edmond Dik Lung Ma

doi:10.1039/c6tb02316a

Iridium(III) complexes with 1,10-phenanthroline-based N^N ligands as highly selective luminescent G-quadruplex probes and application for switch-on ribonuclease H detection

Lihua Lu^*
, Wanhe Wang
, Chao Yang
, Tian Shu Kang
, Chung Hang Leung^*
, Edmond Dik Lung Ma^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

40 Citations (Scopus)

Abstract

A systematic study was performed to investigate the relationship between molecular structure and G-quadruplex sensing ability for a series of structurally related cyclometallated iridium(iii) complexes. The novel complex 7 was used to construct a label-free luminescent switch-on G-quadruplex-based assay for RNase H activity in buffered solution. The constructed detection platform was simple, low-cost and highly sensitive for RNase H, and functioned effectively in the presence of cellular debris.

Original language	English
Pages (from-to)	6791-6796
Number of pages	6
Journal	Journal of Materials Chemistry B
Volume	4
Issue number	42
Early online date	29 Sept 2016
DOIs	https://doi.org/10.1039/c6tb02316a
Publication status	Published - 14 Nov 2016

Access to Document

10.1039/c6tb02316a

Cite this

@article{95e5d565b2694281836d0283d911162c,

title = "Iridium(III) complexes with 1,10-phenanthroline-based N\textasciicircum{}N ligands as highly selective luminescent G-quadruplex probes and application for switch-on ribonuclease H detection",

abstract = "A systematic study was performed to investigate the relationship between molecular structure and G-quadruplex sensing ability for a series of structurally related cyclometallated iridium(iii) complexes. The novel complex 7 was used to construct a label-free luminescent switch-on G-quadruplex-based assay for RNase H activity in buffered solution. The constructed detection platform was simple, low-cost and highly sensitive for RNase H, and functioned effectively in the presence of cellular debris.",

author = "Lihua Lu and Wanhe Wang and Chao Yang and Kang, \{Tian Shu\} and Leung, \{Chung Hang\} and Ma, \{Edmond Dik Lung\}",

note = "Funding Information: This work is supported by Research Foundation for Distinguished Scholars of Qingdao Agricultural University (663-1116010), Hong Kong Baptist University (FRG2/15-16/002), the Health and Medical Research Fund (HMRF/14130522), the Research Grants Council (HKBU/12301115, HKBU/204612 and HKBU/201913), the French Agence Nationale de la Recherche/Research Grants Council Joint Research Scheme (AHKBU201/12; Oligoswitch ANR-12-IS07-0001), the National Natural Science Foundation of China (21575121), the Guangdong Province Natural Science Foundation (2015A030313816), the Hong Kong Baptist University Century Club Sponsorship Scheme 2016, the Science and Technology Development Fund, Macao SAR (098/2014/A2), and the University of Macau (MYRG2015-00137-ICMS-QRCM and MRG044/LCH/2015/ICMS).",

year = "2016",

month = nov,

day = "14",

doi = "10.1039/c6tb02316a",

language = "English",

volume = "4",

pages = "6791--6796",

journal = "Journal of Materials Chemistry B",

issn = "2050-750X",

publisher = "Royal Society of Chemistry",

number = "42",

}

Iridium(III) complexes with 1,10-phenanthroline-based N^N ligands as highly selective luminescent G-quadruplex probes and application for switch-on ribonuclease H detection. / Lu, Lihua; Wang, Wanhe; Yang, Chao et al.
In: Journal of Materials Chemistry B, Vol. 4, No. 42, 14.11.2016, p. 6791-6796.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Iridium(III) complexes with 1,10-phenanthroline-based N^N ligands as highly selective luminescent G-quadruplex probes and application for switch-on ribonuclease H detection

AU - Lu, Lihua

AU - Wang, Wanhe

AU - Yang, Chao

AU - Kang, Tian Shu

AU - Leung, Chung Hang

AU - Ma, Edmond Dik Lung

N1 - Funding Information: This work is supported by Research Foundation for Distinguished Scholars of Qingdao Agricultural University (663-1116010), Hong Kong Baptist University (FRG2/15-16/002), the Health and Medical Research Fund (HMRF/14130522), the Research Grants Council (HKBU/12301115, HKBU/204612 and HKBU/201913), the French Agence Nationale de la Recherche/Research Grants Council Joint Research Scheme (AHKBU201/12; Oligoswitch ANR-12-IS07-0001), the National Natural Science Foundation of China (21575121), the Guangdong Province Natural Science Foundation (2015A030313816), the Hong Kong Baptist University Century Club Sponsorship Scheme 2016, the Science and Technology Development Fund, Macao SAR (098/2014/A2), and the University of Macau (MYRG2015-00137-ICMS-QRCM and MRG044/LCH/2015/ICMS).

PY - 2016/11/14

Y1 - 2016/11/14

N2 - A systematic study was performed to investigate the relationship between molecular structure and G-quadruplex sensing ability for a series of structurally related cyclometallated iridium(iii) complexes. The novel complex 7 was used to construct a label-free luminescent switch-on G-quadruplex-based assay for RNase H activity in buffered solution. The constructed detection platform was simple, low-cost and highly sensitive for RNase H, and functioned effectively in the presence of cellular debris.

AB - A systematic study was performed to investigate the relationship between molecular structure and G-quadruplex sensing ability for a series of structurally related cyclometallated iridium(iii) complexes. The novel complex 7 was used to construct a label-free luminescent switch-on G-quadruplex-based assay for RNase H activity in buffered solution. The constructed detection platform was simple, low-cost and highly sensitive for RNase H, and functioned effectively in the presence of cellular debris.

UR - https://www.scopus.com/pages/publications/84993940466

U2 - 10.1039/c6tb02316a

DO - 10.1039/c6tb02316a

M3 - Journal article

AN - SCOPUS:84993940466

SN - 2050-750X

VL - 4

SP - 6791

EP - 6796

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 42

ER -

Iridium(III) complexes with 1,10-phenanthroline-based N^N ligands as highly selective luminescent G-quadruplex probes and application for switch-on ribonuclease H detection

Abstract

Access to Document

Other files and links

Fingerprint

Cite this