Iridium-based probe for luminescent nitric oxide monitoring in live cells

Chun Wu; Ke Jia Wu; Tian Shu Kang; Hui Min David Wang; Chung Hang Leung; Jin Biao Liu; Dik Lung Ma

doi:10.1038/s41598-018-30991-9

Iridium-based probe for luminescent nitric oxide monitoring in live cells

Chun Wu
, Ke Jia Wu
, Tian Shu Kang
, Hui Min David Wang
, Chung Hang Leung
, Jin Biao Liu^*
, Dik Lung Ma

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

23 Citations (Scopus)

Abstract

Nitric oxide (NO) is an intra- and extracellular messenger with important functions during human physiology process. A long-lived luminescent iridium(III) complex probe 1 has been designed and synthesized for the monitoring of NO controllably released from sodium nitroprusside (SNP). Probe 1 displayed a 15-fold switch-on luminescence in the presence of SNP at 580 nm. The probe exhibited a linear response towards SNP between 5 to 25 μM with detection limit at 0.18 μM. Importantly, the luminescent switch-on detection of NO in HeLa cells was demonstrated. Overall, complex 1 has the potential to be applied for NO tracing in complicated cellular environment.

Original language	English
Article number	12467
Journal	Scientific Reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-30991-9
Publication status	Published - 1 Dec 2018

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title = "Iridium-based probe for luminescent nitric oxide monitoring in live cells",

abstract = "Nitric oxide (NO) is an intra- and extracellular messenger with important functions during human physiology process. A long-lived luminescent iridium(III) complex probe 1 has been designed and synthesized for the monitoring of NO controllably released from sodium nitroprusside (SNP). Probe 1 displayed a 15-fold switch-on luminescence in the presence of SNP at 580 nm. The probe exhibited a linear response towards SNP between 5 to 25 μM with detection limit at 0.18 μM. Importantly, the luminescent switch-on detection of NO in HeLa cells was demonstrated. Overall, complex 1 has the potential to be applied for NO tracing in complicated cellular environment.",

author = "Chun Wu and Wu, \{Ke Jia\} and Kang, \{Tian Shu\} and Wang, \{Hui Min David\} and Leung, \{Chung Hang\} and Liu, \{Jin Biao\} and Ma, \{Dik Lung\}",

note = "Funding Information: This work is supported by Hong Kong Baptist University (FRG2/16-17/007, FRG2/17-18/003), the Health and Medical Research Fund (HMRF/14150561), the Research Grants Council (HKBU/12301115), the National Natural Science Foundation of China (21575121, 21775131, 21628502), the Hong Kong Baptist University Century Club Sponsorship Scheme 2018, the Interdisciplinary Research Matching Scheme (RC-IRMS/15-16/03), Innovation and Technology Fund (ITS/260/16FX), Collaborative Research Fund (C5026-16G), Matching Proof of Concept Fund (MPCF-001-2017/18), the Science and Technology Development Fund, Macao SAR (077/2016/ A2), the University of Macau (MYRG2016-00151-ICMS-QRCM), and the Natural Science Foundation of Jiangxi Province (20171BAB213008).",

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doi = "10.1038/s41598-018-30991-9",

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AU - Wu, Chun

AU - Wu, Ke Jia

AU - Kang, Tian Shu

AU - Wang, Hui Min David

AU - Leung, Chung Hang

AU - Liu, Jin Biao

AU - Ma, Dik Lung

N1 - Funding Information: This work is supported by Hong Kong Baptist University (FRG2/16-17/007, FRG2/17-18/003), the Health and Medical Research Fund (HMRF/14150561), the Research Grants Council (HKBU/12301115), the National Natural Science Foundation of China (21575121, 21775131, 21628502), the Hong Kong Baptist University Century Club Sponsorship Scheme 2018, the Interdisciplinary Research Matching Scheme (RC-IRMS/15-16/03), Innovation and Technology Fund (ITS/260/16FX), Collaborative Research Fund (C5026-16G), Matching Proof of Concept Fund (MPCF-001-2017/18), the Science and Technology Development Fund, Macao SAR (077/2016/ A2), the University of Macau (MYRG2016-00151-ICMS-QRCM), and the Natural Science Foundation of Jiangxi Province (20171BAB213008).

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N2 - Nitric oxide (NO) is an intra- and extracellular messenger with important functions during human physiology process. A long-lived luminescent iridium(III) complex probe 1 has been designed and synthesized for the monitoring of NO controllably released from sodium nitroprusside (SNP). Probe 1 displayed a 15-fold switch-on luminescence in the presence of SNP at 580 nm. The probe exhibited a linear response towards SNP between 5 to 25 μM with detection limit at 0.18 μM. Importantly, the luminescent switch-on detection of NO in HeLa cells was demonstrated. Overall, complex 1 has the potential to be applied for NO tracing in complicated cellular environment.

AB - Nitric oxide (NO) is an intra- and extracellular messenger with important functions during human physiology process. A long-lived luminescent iridium(III) complex probe 1 has been designed and synthesized for the monitoring of NO controllably released from sodium nitroprusside (SNP). Probe 1 displayed a 15-fold switch-on luminescence in the presence of SNP at 580 nm. The probe exhibited a linear response towards SNP between 5 to 25 μM with detection limit at 0.18 μM. Importantly, the luminescent switch-on detection of NO in HeLa cells was demonstrated. Overall, complex 1 has the potential to be applied for NO tracing in complicated cellular environment.

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SN - 2045-2322

VL - 8

JO - Scientific Reports

JF - Scientific Reports

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ER -

Iridium-based probe for luminescent nitric oxide monitoring in live cells

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