OsNAC2 regulates seed dormancy and germination in rice by inhibiting ABA catabolism

Fankai Zhao; Qun Ma; Yingjiang Li; Meihe Jiang; Zhongjing Zhou; Shuan Meng; Yan Peng; Jianhua Zhang; Nenghui Ye; Bohan Liu

doi:10.1016/j.bbrc.2023.10.024

OsNAC2 regulates seed dormancy and germination in rice by inhibiting ABA catabolism

Fankai Zhao, Qun Ma, Yingjiang Li, Meihe Jiang, Zhongjing Zhou, Shuan Meng, Yan Peng, Jianhua Zhang^*, Nenghui Ye^*, Bohan Liu^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

1 Citation (Scopus)

Abstract

Seed dormancy and germination determine the beginning of the life cycle of plants, and the phytohormone ABA plays a crucial role in regulation of seed dormancy and germination. However, the upstream regulatory mechanism of ABA metabolism during dormancy releasing is still remain elusive. In this paper, we present a novel mechanism of OsNAC2 in controlling ABA metabolism and regulation of seed dormancy. OsNAC2 highly expressed during seed development and germination, and overexpression of OsNAC2 strengthened seed dormancy and suppressed germination. Moreover, exogenous phytohormone treatment showed that OsNAC2 acted upstream of GA signaling and downstream of ABA signaling. Additionally, overexpression of OsNAC2 inhibited ABA degradation and increased ABA content during early germination. Further molecular analysis revealed that OsNAC2 directly bound to the ABA metabolism genes promoter and inhibits their transcription in rice protoplasts. These finding could help us explain the genetic regulation mechanism of ABA metabolism during dormancy release and germination in rice.

Original language	English
Pages (from-to)	335-342
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	682
DOIs	https://doi.org/10.1016/j.bbrc.2023.10.024
Publication status	Published - 19 Nov 2023

Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

User-Defined Keywords

ABA metabolism
CRISPR/Cas9
Dormancy
Germination
OsNAC2
Rice

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2023.10.024

Cite this

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title = "OsNAC2 regulates seed dormancy and germination in rice by inhibiting ABA catabolism",

abstract = "Seed dormancy and germination determine the beginning of the life cycle of plants, and the phytohormone ABA plays a crucial role in regulation of seed dormancy and germination. However, the upstream regulatory mechanism of ABA metabolism during dormancy releasing is still remain elusive. In this paper, we present a novel mechanism of OsNAC2 in controlling ABA metabolism and regulation of seed dormancy. OsNAC2 highly expressed during seed development and germination, and overexpression of OsNAC2 strengthened seed dormancy and suppressed germination. Moreover, exogenous phytohormone treatment showed that OsNAC2 acted upstream of GA signaling and downstream of ABA signaling. Additionally, overexpression of OsNAC2 inhibited ABA degradation and increased ABA content during early germination. Further molecular analysis revealed that OsNAC2 directly bound to the ABA metabolism genes promoter and inhibits their transcription in rice protoplasts. These finding could help us explain the genetic regulation mechanism of ABA metabolism during dormancy release and germination in rice.",

keywords = "ABA metabolism, CRISPR/Cas9, Dormancy, Germination, OsNAC2, Rice",

author = "Fankai Zhao and Qun Ma and Yingjiang Li and Meihe Jiang and Zhongjing Zhou and Shuan Meng and Yan Peng and Jianhua Zhang and Nenghui Ye and Bohan Liu",

note = "Funding information: This work was supported by the Natural Science Foundation of Hunan Province (2021JJ30349), the National Natural Science Foundation of China (32301739, 32171927, 31971924), the Hong Kong Research Grant Council (AoE/M-05/12, AoE/M-403/16, GRF12103219, 12103220, 12101722) and Foundation of Hunan Educational Committee (21B0205). Publisher Copyright: {\textcopyright} 2023 Elsevier Inc.",

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day = "19",

doi = "10.1016/j.bbrc.2023.10.024",

language = "English",

volume = "682",

pages = "335--342",

journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - OsNAC2 regulates seed dormancy and germination in rice by inhibiting ABA catabolism

AU - Zhao, Fankai

AU - Ma, Qun

AU - Li, Yingjiang

AU - Jiang, Meihe

AU - Zhou, Zhongjing

AU - Meng, Shuan

AU - Peng, Yan

AU - Zhang, Jianhua

AU - Ye, Nenghui

AU - Liu, Bohan

N1 - Funding information: This work was supported by the Natural Science Foundation of Hunan Province (2021JJ30349), the National Natural Science Foundation of China (32301739, 32171927, 31971924), the Hong Kong Research Grant Council (AoE/M-05/12, AoE/M-403/16, GRF12103219, 12103220, 12101722) and Foundation of Hunan Educational Committee (21B0205). Publisher Copyright: © 2023 Elsevier Inc.

PY - 2023/11/19

Y1 - 2023/11/19

N2 - Seed dormancy and germination determine the beginning of the life cycle of plants, and the phytohormone ABA plays a crucial role in regulation of seed dormancy and germination. However, the upstream regulatory mechanism of ABA metabolism during dormancy releasing is still remain elusive. In this paper, we present a novel mechanism of OsNAC2 in controlling ABA metabolism and regulation of seed dormancy. OsNAC2 highly expressed during seed development and germination, and overexpression of OsNAC2 strengthened seed dormancy and suppressed germination. Moreover, exogenous phytohormone treatment showed that OsNAC2 acted upstream of GA signaling and downstream of ABA signaling. Additionally, overexpression of OsNAC2 inhibited ABA degradation and increased ABA content during early germination. Further molecular analysis revealed that OsNAC2 directly bound to the ABA metabolism genes promoter and inhibits their transcription in rice protoplasts. These finding could help us explain the genetic regulation mechanism of ABA metabolism during dormancy release and germination in rice.

AB - Seed dormancy and germination determine the beginning of the life cycle of plants, and the phytohormone ABA plays a crucial role in regulation of seed dormancy and germination. However, the upstream regulatory mechanism of ABA metabolism during dormancy releasing is still remain elusive. In this paper, we present a novel mechanism of OsNAC2 in controlling ABA metabolism and regulation of seed dormancy. OsNAC2 highly expressed during seed development and germination, and overexpression of OsNAC2 strengthened seed dormancy and suppressed germination. Moreover, exogenous phytohormone treatment showed that OsNAC2 acted upstream of GA signaling and downstream of ABA signaling. Additionally, overexpression of OsNAC2 inhibited ABA degradation and increased ABA content during early germination. Further molecular analysis revealed that OsNAC2 directly bound to the ABA metabolism genes promoter and inhibits their transcription in rice protoplasts. These finding could help us explain the genetic regulation mechanism of ABA metabolism during dormancy release and germination in rice.

KW - ABA metabolism

KW - CRISPR/Cas9

KW - Dormancy

KW - Germination

KW - OsNAC2

KW - Rice

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U2 - 10.1016/j.bbrc.2023.10.024

DO - 10.1016/j.bbrc.2023.10.024

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AN - SCOPUS:85173643081

SN - 0006-291X

VL - 682

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

ER -

OsNAC2 regulates seed dormancy and germination in rice by inhibiting ABA catabolism

Abstract

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