OsNAC2 regulates seed dormancy and germination in rice by inhibiting ABA catabolism

Fankai Zhao, Qun Ma, Yingjiang Li, Meihe Jiang, Zhongjing Zhou, Shuan Meng, Yan Peng, Jianhua Zhang*, Nenghui Ye*, Bohan Liu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

1 Citation (Scopus)


Seed dormancy and germination determine the beginning of the life cycle of plants, and the phytohormone ABA plays a crucial role in regulation of seed dormancy and germination. However, the upstream regulatory mechanism of ABA metabolism during dormancy releasing is still remain elusive. In this paper, we present a novel mechanism of OsNAC2 in controlling ABA metabolism and regulation of seed dormancy. OsNAC2 highly expressed during seed development and germination, and overexpression of OsNAC2 strengthened seed dormancy and suppressed germination. Moreover, exogenous phytohormone treatment showed that OsNAC2 acted upstream of GA signaling and downstream of ABA signaling. Additionally, overexpression of OsNAC2 inhibited ABA degradation and increased ABA content during early germination. Further molecular analysis revealed that OsNAC2 directly bound to the ABA metabolism genes promoter and inhibits their transcription in rice protoplasts. These finding could help us explain the genetic regulation mechanism of ABA metabolism during dormancy release and germination in rice.

Original languageEnglish
Pages (from-to)335-342
Number of pages8
JournalBiochemical and Biophysical Research Communications
Publication statusPublished - 19 Nov 2023

Scopus Subject Areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

User-Defined Keywords

  • ABA metabolism
  • CRISPR/Cas9
  • Dormancy
  • Germination
  • OsNAC2
  • Rice


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