Ion-pair chromatographic separation of water-soluble gold monolayer-protected clusters

Martin M F CHOI; Alicia D. Douglas; Royce W. Murray

doi:10.1021/ac052167m

Ion-pair chromatographic separation of water-soluble gold monolayer-protected clusters

Martin M F CHOI, Alicia D. Douglas, Royce W. Murray^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

70 Citations (Scopus)

Abstract

We demonstrate the efficacy of ion-pair chromatography for separations of samples of charged, polydisperse, water-soluble gold nanoparticles protected by monolayers of N-acetyl-L-cysteine and of tiopronin ligands. These nanoparticle mixtures have 1-2-nm-diameter Au core sizes as estimated from UV-visible spectra of the separated components. This size range encompasses the transition from bulk metal to molecular properties. The nanoparticle mixtures were resolved, the smallest nano-particles eluting first, on an octadecylsilyl (C18) column using isocratic elution with a methanol/water mobile phase containing tetrabutylammonium fluoride (Bu₄N⁺F^-) and phosphate buffer. The column retention increases with Bu₄N ⁺F^- concentration, lowered pH, and decreasing methanol volume fraction. The retention mechanism is dominated by ion-pairing in either the mobile phase or at the stationary/mobile-phase interface. Size exclusion effects, used in many previous nanoparticle separations, are insignificant.

Original language	English
Pages (from-to)	2779-2785
Number of pages	7
Journal	Analytical Chemistry
Volume	78
Issue number	8
DOIs	https://doi.org/10.1021/ac052167m
Publication status	Published - 15 Apr 2006

Scopus Subject Areas

Analytical Chemistry

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title = "Ion-pair chromatographic separation of water-soluble gold monolayer-protected clusters",

abstract = "We demonstrate the efficacy of ion-pair chromatography for separations of samples of charged, polydisperse, water-soluble gold nanoparticles protected by monolayers of N-acetyl-L-cysteine and of tiopronin ligands. These nanoparticle mixtures have 1-2-nm-diameter Au core sizes as estimated from UV-visible spectra of the separated components. This size range encompasses the transition from bulk metal to molecular properties. The nanoparticle mixtures were resolved, the smallest nano-particles eluting first, on an octadecylsilyl (C18) column using isocratic elution with a methanol/water mobile phase containing tetrabutylammonium fluoride (Bu4N+F-) and phosphate buffer. The column retention increases with Bu4N +F- concentration, lowered pH, and decreasing methanol volume fraction. The retention mechanism is dominated by ion-pairing in either the mobile phase or at the stationary/mobile-phase interface. Size exclusion effects, used in many previous nanoparticle separations, are insignificant.",

author = "CHOI, {Martin M F} and Douglas, {Alicia D.} and Murray, {Royce W.}",

note = "This research was supported in part by grants from the National Science Foundation and the Office of Naval Research. M.M.F.C. also acknowledges the support of Hong Kong Baptist University by a 12-month sabbatical program. ",

year = "2006",

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doi = "10.1021/ac052167m",

language = "English",

volume = "78",

pages = "2779--2785",

journal = "Analytical Chemistry",

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T1 - Ion-pair chromatographic separation of water-soluble gold monolayer-protected clusters

AU - CHOI, Martin M F

AU - Douglas, Alicia D.

AU - Murray, Royce W.

N1 - This research was supported in part by grants from the National Science Foundation and the Office of Naval Research. M.M.F.C. also acknowledges the support of Hong Kong Baptist University by a 12-month sabbatical program.

PY - 2006/4/15

Y1 - 2006/4/15

N2 - We demonstrate the efficacy of ion-pair chromatography for separations of samples of charged, polydisperse, water-soluble gold nanoparticles protected by monolayers of N-acetyl-L-cysteine and of tiopronin ligands. These nanoparticle mixtures have 1-2-nm-diameter Au core sizes as estimated from UV-visible spectra of the separated components. This size range encompasses the transition from bulk metal to molecular properties. The nanoparticle mixtures were resolved, the smallest nano-particles eluting first, on an octadecylsilyl (C18) column using isocratic elution with a methanol/water mobile phase containing tetrabutylammonium fluoride (Bu4N+F-) and phosphate buffer. The column retention increases with Bu4N +F- concentration, lowered pH, and decreasing methanol volume fraction. The retention mechanism is dominated by ion-pairing in either the mobile phase or at the stationary/mobile-phase interface. Size exclusion effects, used in many previous nanoparticle separations, are insignificant.

AB - We demonstrate the efficacy of ion-pair chromatography for separations of samples of charged, polydisperse, water-soluble gold nanoparticles protected by monolayers of N-acetyl-L-cysteine and of tiopronin ligands. These nanoparticle mixtures have 1-2-nm-diameter Au core sizes as estimated from UV-visible spectra of the separated components. This size range encompasses the transition from bulk metal to molecular properties. The nanoparticle mixtures were resolved, the smallest nano-particles eluting first, on an octadecylsilyl (C18) column using isocratic elution with a methanol/water mobile phase containing tetrabutylammonium fluoride (Bu4N+F-) and phosphate buffer. The column retention increases with Bu4N +F- concentration, lowered pH, and decreasing methanol volume fraction. The retention mechanism is dominated by ion-pairing in either the mobile phase or at the stationary/mobile-phase interface. Size exclusion effects, used in many previous nanoparticle separations, are insignificant.

UR - http://www.scopus.com/inward/record.url?scp=33646140372&partnerID=8YFLogxK

U2 - 10.1021/ac052167m

DO - 10.1021/ac052167m

M3 - Journal article

AN - SCOPUS:33646140372

SN - 0003-2700

VL - 78

SP - 2779

EP - 2785

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 8

ER -

Ion-pair chromatographic separation of water-soluble gold monolayer-protected clusters

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