TY - JOUR
T1 - Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings
AU - Jiang, Mingyi
AU - Zhang, Jianhua
N1 - Funding Information:
Acknowledgements We are grateful for grants obtained from the Faculty Research Grants of Hong Kong Baptist University, the Research Grants Council of Hong Kong University Grants Council and Area of Excellence for Plant and Fungal Biotechnology. M.J. is grateful for a postgraduate studentship from Hong Kong Baptist University.
PY - 2002
Y1 - 2002
N2 - The roles of the plasma-membrane (PM) NADPH oxidase in abscisic acid (ABA)- and water stress-induced antioxidant defense were investigated in leaves of maize (Zea mays L.) seedlings. Treatment by exogenous ABA (100 μM ABA) or osmotic stress (-0.7 MPa induced by polyethylene glycol) significantly increased the activity of the PM NADPH oxidase, the production of leaf O2-, the activities of several antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase), and the contents of antioxidant metabolites (ascorbate and reduced glutathione). Pretreatment with three different inhibitors of NADPH oxidase (diphenylene iodonium, imidazole and pyridine) or an inhibitor of ABA biosynthesis (tungstate) reduced the increase in the activity of the PM NADPH oxidase and the production of leaf O2-, and the capacity of antioxidant defense systems mediated by ABA. The inhibitory effects above caused by tungstate were reversed by exogenous ABA. These data indicate that NADPH oxidase is involved m the ABA-induced production of active oxygen species (AOS), and our results depict a minimal chain of events initiated by water stress-reduced ABA accumulation, which then triggers the production of AOS by membrane-bound NADPH oxidase, resulting in the induction of antioxidant defense systems against oxidative damage in plants.
AB - The roles of the plasma-membrane (PM) NADPH oxidase in abscisic acid (ABA)- and water stress-induced antioxidant defense were investigated in leaves of maize (Zea mays L.) seedlings. Treatment by exogenous ABA (100 μM ABA) or osmotic stress (-0.7 MPa induced by polyethylene glycol) significantly increased the activity of the PM NADPH oxidase, the production of leaf O2-, the activities of several antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase), and the contents of antioxidant metabolites (ascorbate and reduced glutathione). Pretreatment with three different inhibitors of NADPH oxidase (diphenylene iodonium, imidazole and pyridine) or an inhibitor of ABA biosynthesis (tungstate) reduced the increase in the activity of the PM NADPH oxidase and the production of leaf O2-, and the capacity of antioxidant defense systems mediated by ABA. The inhibitory effects above caused by tungstate were reversed by exogenous ABA. These data indicate that NADPH oxidase is involved m the ABA-induced production of active oxygen species (AOS), and our results depict a minimal chain of events initiated by water stress-reduced ABA accumulation, which then triggers the production of AOS by membrane-bound NADPH oxidase, resulting in the induction of antioxidant defense systems against oxidative damage in plants.
KW - Abscisic acid
KW - Active oxygen species
KW - Antioxidant defense system
KW - NADPH oxidase
KW - Water stress
KW - Zea
UR - http://www.scopus.com/inward/record.url?scp=0036943993&partnerID=8YFLogxK
U2 - 10.1007/s00425-002-0829-y
DO - 10.1007/s00425-002-0829-y
M3 - Journal article
C2 - 12355163
AN - SCOPUS:0036943993
SN - 0032-0935
VL - 215
SP - 1022
EP - 1030
JO - Planta
JF - Planta
IS - 6
ER -