Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings

Mingyi Jiang, Jianhua ZHANG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

251 Citations (Scopus)

Abstract

The roles of the plasma-membrane (PM) NADPH oxidase in abscisic acid (ABA)- and water stress-induced antioxidant defense were investigated in leaves of maize (Zea mays L.) seedlings. Treatment by exogenous ABA (100 μM ABA) or osmotic stress (-0.7 MPa induced by polyethylene glycol) significantly increased the activity of the PM NADPH oxidase, the production of leaf O2-, the activities of several antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase), and the contents of antioxidant metabolites (ascorbate and reduced glutathione). Pretreatment with three different inhibitors of NADPH oxidase (diphenylene iodonium, imidazole and pyridine) or an inhibitor of ABA biosynthesis (tungstate) reduced the increase in the activity of the PM NADPH oxidase and the production of leaf O2-, and the capacity of antioxidant defense systems mediated by ABA. The inhibitory effects above caused by tungstate were reversed by exogenous ABA. These data indicate that NADPH oxidase is involved m the ABA-induced production of active oxygen species (AOS), and our results depict a minimal chain of events initiated by water stress-reduced ABA accumulation, which then triggers the production of AOS by membrane-bound NADPH oxidase, resulting in the induction of antioxidant defense systems against oxidative damage in plants.

Original languageEnglish
Pages (from-to)1022-1030
Number of pages9
JournalPlanta
Volume215
Issue number6
DOIs
Publication statusPublished - 2002

Scopus Subject Areas

  • Genetics
  • Plant Science

User-Defined Keywords

  • Abscisic acid
  • Active oxygen species
  • Antioxidant defense system
  • NADPH oxidase
  • Water stress
  • Zea

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