Investigation of the effects of wall adsorption of the visualization agent on baseline noise characteristics for indirect UV detection in capillary electrophoresis

Tom M.H. Choy, Carmen W K HUIE*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

New insights into the effects of the wall adsorption of the visualization agent on baseline noise characteristics in indirect UV detection in capillary electrophoresis were provided. When compared to relatively small, hydrophilic cationic (1-(4-pyridyl)pyridinium chloride hydrochloride) or anionic chromophores (bromide and benzoate), the use of a UV-absorbing long-chain cationic surfactant (benzyldimethylhexadecylammonium chloride) as a probe (visualization agent) for studying baseline noise characteristics as a function of various experimental conditions, such as applied voltage, pH or buffer composition, was found to provide noise data that were very different under certain conditions. For cationic visualization agents that have a strong tendency of adsorbing onto the capillary surfaces due to electrostatic and/or hydrophobic interactions, it appeared that the interplay of both thermal as well as adsorption/desorption events occurring within the capillary is likely responsible for a fluctuation of the visualization agent concentration at the detector.

Original languageEnglish
Pages (from-to)3040-3048
Number of pages9
JournalElectrophoresis
Volume24
Issue number17
DOIs
Publication statusPublished - Sep 2003

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

User-Defined Keywords

  • Capillary electrophoresis
  • Indirect UV detection
  • Noise characteristic
  • Visualization agent
  • Wall adsorption

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