1. 1. Potent hemagglutinating activity was detected in seed extracts of 13 out of the 14 Trichosanthes species examined. T. pedata seed extract demonstrated the lowest hemagglutinating activity. 2. 2. d(+) galactose, d(+) galactosamine, d(+) raffinose and d(+) fucose but not l(-) fucose inhibited the hemagglutinating activity of these seed extracts. 3. 3. Lectin was purified from Trichosanthes kirilowii seed extract by a procedure involving solvent fractionation, gel filtration on Sephadex G-75 and affinity chromatography on Lacto-Sepharose. 4. 4. The biochemical properties of T. kirilowii seed lectin were compared with those of T. kirilowii tuber isolectins. Their sugar binding specificities and amino acid compositions were similar. However, there was a difference in molecular weight.
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