Introgressive hybridization between two non-native apple snails in China: widespread hybridization and homogenization in egg morphology

Qian Qian Yang, Chao He, Guang Fu Liu, Chuan Lin Yin, Yi Peng Xu, Su Wen Liu, Jianwen QIU, Xiao Ping Yu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Apple snails from the genus Pomacea have spread widely in paddy fields and other wetlands of southern China since their introduction in the 1980s. Pomacea spp. are commonly identified using mitochondrial COI sequences. However, sequencing the nuclear elongation factor 1-alpha (EF1α) gene revealed genetic introgression between field populations of P. canaliculata and P. maculata, which produce surviving hybrids in laboratory crossbreeding experiments. RESULTS: In this study, we sequenced 1054 EF1α clones to design specific primers and established a fast and accurate multiplex polymerase chain reaction (PCR) method for genotyping EF1α. Combined with genotyping P. canaliculata and P. maculata based on mitochondrial COI and nuclear EF1α, we revealed the genetic introgression patterns of 30 apple snail populations in China. Purebred and hybrid individuals of P. canaliculata were widely distributed, while pure maculata-EF1α type was detected only in a few individuals identified as P. canaliculata based on COI sequences. Each egg clutch had one to three genetic patterns, indicating multiple paternity or segregation in the progeny of hybrids. The higher percentages of hybrids in both wild populations and progeny than the homozygotes indicated a potential heterosis in the apple snail populations. Additionally, egg size and clutch size of the apple snails became homogeneous among the non-native populations exhibiting introgression hybridization. CONCLUSION: Our findings emphasize the value of apple snails as a model to study the mechanisms and impacts of introgressive hybridization on fitness traits.

Original languageEnglish
Pages (from-to)4231-4239
Number of pages9
JournalPest Management Science
Volume76
Issue number12
DOIs
Publication statusPublished - 1 Dec 2020

Scopus Subject Areas

  • Agronomy and Crop Science
  • Insect Science

User-Defined Keywords

  • egg size
  • gastropods
  • hybrids
  • invasive species
  • morphology
  • specific primers

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