TY - JOUR
T1 - Intraneuronal Nanotracers in Axonal Transport
AU - Yi, Zhigao
AU - All, Angelo H.
AU - Liu, Yuxia
AU - Liu, Xiaogang
N1 - This work was supported by the National Key R&D Program of China (2024YFC2418700), the Suzhou Basic Research Pilot Project (SSD2024011), and the Gusu Innovation and Entrepreneurship Leading Talent Plan (ZXL2024364), the National Research Foundation, Prime Minister’s Office, Singapore under the NRF Investigatorship Programme (Award No. NRF-NRFI05-2019-0003), NUS NANONASH Programme (NUHSRO/2020/002/NanoNash/LOA; R143000B43114).
Publisher Copyright:
© 2025 American Chemical Society
PY - 2025/10/14
Y1 - 2025/10/14
N2 - Axonal transport is fundamental to neuronal function, ensuring the movement of organelles, proteins, and other cellular components necessary for growth, signaling, and repair. Disruptions in this transport system are implicated in various neurodegenerative and neurodevelopmental disorders. Recent advances in tracking methods, both in vivo and in vitro, have deepened our understanding of transport defects, their molecular mechanisms, and their potential applications in diagnostics and therapeutics. This review discusses key scientific and technical breakthroughs in axonal transport visualization, revisiting the core principles of transport machinery. We examine critical components involved in axonal transport, including microtubules, motor proteins and their adaptors, and organelle cargoes. Additionally, we highlight recent developments in nanomaterial-based tracing methods, present notable examples, and outline future challenges and research directions.
AB - Axonal transport is fundamental to neuronal function, ensuring the movement of organelles, proteins, and other cellular components necessary for growth, signaling, and repair. Disruptions in this transport system are implicated in various neurodegenerative and neurodevelopmental disorders. Recent advances in tracking methods, both in vivo and in vitro, have deepened our understanding of transport defects, their molecular mechanisms, and their potential applications in diagnostics and therapeutics. This review discusses key scientific and technical breakthroughs in axonal transport visualization, revisiting the core principles of transport machinery. We examine critical components involved in axonal transport, including microtubules, motor proteins and their adaptors, and organelle cargoes. Additionally, we highlight recent developments in nanomaterial-based tracing methods, present notable examples, and outline future challenges and research directions.
KW - axonal tracking
KW - cargo transport
KW - microscopic imaging
KW - microtubule
KW - motor protein
KW - nanotracer
KW - neurodegenerative disease
KW - photoluminescence
KW - upconversion nanoparticle
UR - http://www.scopus.com/inward/record.url?scp=105018688036&partnerID=8YFLogxK
U2 - 10.1021/acsnano.5c13559
DO - 10.1021/acsnano.5c13559
M3 - Journal article
C2 - 41026858
AN - SCOPUS:105018688036
SN - 1936-0851
VL - 19
SP - 35306
EP - 35321
JO - ACS Nano
JF - ACS Nano
IS - 40
ER -